中国HIV暴露未感者CD4+T淋巴细胞的体外抗HIV活性  

Resistance to HIV-1 Infection of CD4+T Lymphocytes in vitro from Chinese HIV-1 Exposed Seronegative Individuals

作　　者：苏艳丽[1] 尚红[1] 刘静[1] 王树祥[1] 耿文清[1] 崔华露[1] 姜拥军[1] 王亚男[1] 张子宁[1] 王亚婷[1] 

机构地区：[1]中国医科大学附属第一医院艾滋病研究中心,沈阳110001

出　　处：《中国医学科学院学报》2006年第5期613-617,共5页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金(30271188);教育部博士点专项(20040159005);辽宁省教育厅科技攻关计划(2012215112)~~

摘　　要：目的了解中国经性途径暴露于人类免疫缺陷病毒(HIV)而未感染者(ESN)的CD4+T淋巴细胞在体外的抗HIV活性,探讨中国ESN的抗HIV感染机制。方法采用微量全血法分离培养HIV感染者的病毒株,密度梯度离心法分离ESN外周血单个核细胞后用MACS磁分选法分选出CD4+T淋巴细胞,与HIV感染者的病毒分离株共培养,检测共培养上清的HIV-1复制动力(p24抗原)。结果ESN组CD4+T淋巴细胞对M嗜性分离毒株的复制动力显著低于健康对照组(P<0.05);ESN组CD4+T淋巴细胞对M嗜性分离毒株的复制动力显著低于T嗜性分离毒株(P<0.05);ESN组CD4+T淋巴细胞对T嗜性分离毒株及实验室毒株的感染能力与健康对照组相比差异无显著性(P>0.05)。结论中国ESN的CD4+T淋巴细胞对M嗜性病毒分离株有一定抵抗作用,可能是经性接触暴露未感者抗HIV感染的主要影响因素。Objective To determine the relative resistance to HIV-1 infection of CD4 ＋ T lymphocytes in HIV-exposed seronegative individuals （ESNs） in China. Methods HIV primary isolates were obtained from peripheral whole blood of HIV-infeeted persons. CD4 ＋ T lymphoeytes of Chinese ESNs were separated from peripheral blood mononuelear cells with magnetic cell sorting （MACS）. The purified CD4 ＋ T lymphocytes were eoeuhured with HIV primary isolates. The p24 level was detected and the culture medium was refreshed every 3 days within 2 weeks. Results For M tropic HIV strains, p24 level was significantly lower in ESN group than in control group （ P 〈 0.05 ） ; for some M tropic HIV strains, even no p24 replicated in ESN group. However, T tropic virus strains had no significant difference between these two groups （ P 〉 0.05 ）. Conclusion CD4 ＋ T lymphoeytes of Chinese ESNs may possess relative resistance to M tropic HIV strains, which may be one of the main influencing factors that result in ESN.

关 键 词：人类免疫缺陷病毒 暴露未感者 M嗜性 T嗜性 

分 类 号：R512.91[医药卫生—内科学]