艾滋病相关淋巴结病理改变及其与淋巴结中CD4+CD25+调节性T细胞的相关性  

Pathology of AIDS-related Lymphadenopathy and Its Relationship to Expression of CD4+CD25+ Regulatory T Cells in Lymphoid Node Tissue

作　　者：郎振为[1] 马佩卿[1] 赵红心[1] 兰孟东[1] 刀文彬[1] 张亮[1] 沈冰[1] 石晓虹[1] 马志春[1] 王培之[1] 张福杰[1] 

机构地区：[1]北京地坛医院病理科,北京100011

出　　处：《中国医学科学院学报》2006年第5期622-625,I0001,共5页Acta Academiae Medicinae Sinicae

基　　金：首都医学发展科研基金(ZD199914)~~

摘　　要：目的探讨艾滋病患者淋巴结组织中CD4+CD25+调节性T细胞的表达、分布及其与病理改变的相关性。方法对22例活检及13例尸体解剖的HIV/AIDS患者的淋巴结进行组织学观察和病理分期,采用免疫组织化学法对淋巴结组织中的CD4+CD25+调节性T细胞特异性标记物FoxP3进行检测。结果35例艾滋病患者的淋巴结中,1～4期分别有5、4、14和12例。所有淋巴结组织中均检测出FoxP3的阳性表达;在1、2期病变淋巴结内,FoxP3阳性细胞数量较多,分布于滤泡间区和副皮质区;3、4期随着淋巴细胞的数量衰竭,阳性细胞数量的减少更加明显。结论艾滋病患者淋巴结中存在有CD4+CD25+调节性T细胞,随着淋巴结病变的进展其数量减少或耗竭。Objective To observe the pathology of AIDS-related lymphadenopathy and its relationship to the expression and distribution of CD4 ＋ CD25 ＋ regulatory T cells in lymphoid node tissue. Methods Totally 22 biopsy and 13 autopsy lymphoid node tissues from HIV-positive patients were examined under microscopy and pathological staging was performed. Specific marker for CD4 ＋ CD25 ＋ regulatory T cells in lymphoid node tissue was detected with anti-Foxp3 monoclonal antibody by immunohistochemistry. Results Among all the 35 specimens, 5, 4, 14, and 12 specimens were histopathologically staged from 1 to 4, respectively. FoxP3 were detected in all lymphoid node tissues. The distribution of FoxP3-positive lymphocytes were mainly in intermediate zone of follicle and cortical area in stages 1 and 2. The counts of FoxP3-positive lymphocytes remarkedly decreased in stages 3 and 4, following depletion of lymphocytes. Conclusions CD4 ＋ CD25 ＋ regulatory T cells exist in lymphoid node tissue of patients with HIV infection. Their amounts decrease or deplete along with the progression of AIDS-related lymphadenopathy.

关 键 词：获得性免疫缺陷综合征 调节性T淋巴细胞 淋巴结 免疫组织化学 

分 类 号：R512.91[医药卫生—内科学]