磁共振灌注成像监测组织工程骨血管化的研究  

Preliminary experimental study of monitoring vascularization of tissue engineered bone with the perfusion weighted MR imaging

作　　者：王学明[1] 裴国献[1] 金丹[1] 魏宽海[1] 江汕[1] 唐光辉[1] 

机构地区：[1]南方医科大学南方医院创伤骨科,广州510515

出　　处：《中华实验外科杂志》2006年第12期1510-1512,共3页Chinese Journal of Experimental Surgery

基　　金：国家高技术发展计划(863计划)重大专项基金资助项目(2003AA205010)

摘　　要：目的探讨磁共振灌注成像在组织工程骨血管化监测中的应用价值。方法成年恒河猴体内制备胫骨20 mm的骨缺损,随机分为5组,A组:β-磷酸三钙(β-TCP)+骨髓基质干细胞(BMSCs)+血管束;B组:β-TCP+血管束;C组:β-TCP+BMSCs;D组:β-TCP;E组:空白对照。术后4、8、12周行磁共振灌注成像检查并计算信号强度-时间(SI-T)曲线的最大线性斜率(SSmax)和基线值(SIbaseline),拍摄恒河猴胫骨X线片并计算其透光度。结果术后4、8、12周A组的SSmax值最高,术后8周与4周相比SSmax有较大幅度的提高(P<0．01)。术后12周A组5个样本的SSmax与X线片透光度呈负相关(r=-1．0,P<0．01)。结论SI-T曲线的SSmax能准确反映组织工程骨的血管化情况,磁共振灌注成像检查具有无创、无辐射、高灵敏度和定量分析的优点。Objective To assess the diagnostic value of perfusion weighted MR imaging technique in monitoring vaseularization of tissue engineered bone. Methods A tibial diaphyseal defect of 20 mm was made in adult rhesus. The monkeys were randomly divided into 5 groups：group A,β-tricalcium phosphate β-TCP）＋ bone marrow stromal cells （BMSCs） ＋ blood vessel bundles; group B, β-TCP ＋ blood vessel bundles; group C,β-TCP ＋ BMSCs; group D, β-TCP ; group E, blank. Perfusion weighted MR imaging and X-ray examinations were carried out at week 4,8 and 12 postopration. The steep slope of the single intensity-time curve （SSm~） and value of baseline （SIbascline） were calculated. Penetrate light degree of X-ray was detected. Results The SSmax in group A was the highest at week 4,8 and 12 postopration. At the postoperative week 8, SSmax had more significant exaltation than at 4th week （P 〈 0.01 ）. The SSmax was negatively related with penetrate light degree of X-ray at 12th week after operation （ r = - 1.0, P 0.05）. Conclusion The SSmax can reflect the vaseularity of tissue engineered bone accurately. Perfusion weighted MR imaging technique has advantages of high sensitivity, quantitative analysis, non-invasion and non-radiation.

关 键 词：磁共振成像 灌注 组织工程 骨 血管化 

分 类 号：R318.0[医药卫生—生物医学工程]