小剂量FK778对大鼠移植肾慢性排斥反应的预防作用  

Effect of low dose of FK778 on the progression of chronic renal allograft rejection in a rat model

作　　者：魏立新 郝博[2] 詹林达[1] 余学清[1] 

机构地区：[1]中山大学附属第一医院肾脏科,广州510000 [2]中山大学附属第一医院器官移植外科,广州510000

出　　处：《中华实验外科杂志》2006年第12期1529-1530,共2页Chinese Journal of Experimental Surgery

摘　　要：目的探讨小剂量FK778对大鼠移植肾慢性排斥反应的预防作用。方法应用显微外科技术制作移植肾慢性排斥反应大鼠模型,将肾移植大鼠分为两组。肾移植后第16周开始治疗组大鼠接受FK778每日5 mg／kg体重灌胃,对照组接受赋形剂。移植后每4周行24 h尿蛋白含量测定,第24周处死大鼠,对移植肾组织行组织学、免疫组织化学及实时定量RT-PCR检测。结果治疗组大鼠蛋白尿、肾组织病理损害程度,淋巴细胞和单核／巨噬细胞浸润程度和对照组比较显著减轻,肾组织生长因子TGF-β基因的表达也减少。结论小剂量FK778能预防大鼠移植肾慢性排斥反应。移植肾组织T淋巴细胞和单核／巨噬细胞浸润减轻,TGF-β生长因子基因表达的减少,可能是其预防同种移植肾慢性排斥反应机制中的重要环节。ObjectiveTo study the effect of low-dose of FK778 in preventing chronic renal allograft rejection in rats. Methods The rat model of chronic renal allograft rejection was made with microsurgery. The recipients were divided into two groups. The recipients in the study group were treated with FK778 at a dose of 5 mg/kg·d^-1 by means of gavage and the controls with vehicle from the 16th week after kidney transplantation. Results After the treatment with FK778, proteinuria, the grade of chronic rejection, glomerulosclerosis and the infiltration of T-lymphocytes and monocytes/macrophages in the study group were significantly decreased and the expression of the TGF-β mRNA in renal tissue was signficantly reduced as compared with the controls. Conclusion Low dose of FK778 can prevent chronic renal allograft rejection. The decreased infiltration of T-lymphocytes and monocytes/macrophages as well as the reduced expression of TGF-β in renal tissue may be involved in the preventive mechanism of chronic renal allograft rejection.

关 键 词：FK778 移植肾 慢性排斥反应 

分 类 号：R699.2[医药卫生—泌尿科学]