维生素B_1和维生素C联合应用对铅染毒小鼠睾丸某些生化和形态指标的影响  

Impacts of combined supplementation with ascorbic acid and thiamine on certain biochemical and morphologic indexes of testes in mice treated by lead

作　　者：张妍[1] 汪春红[1] 梁建成[1] 张春莲[1] 王程强[1] 

机构地区：[1]武汉大学公共卫生学院,武汉430071

出　　处：《卫生研究》2006年第6期731-734,共4页Journal of Hygiene Research

基　　金：湖北省卫生厅医学科研项目(WJ01551)

摘　　要：目的探讨维生素B1、C联合应用对铅染毒小鼠睾丸某些生化和形态指标的影响。方法75只雄性小鼠随机分为空白对照组,0.2%染铅组和维生素高、中、低干预组,每组各15只。并分别在第2、4、6周处死每组各5只小鼠,取其睾丸组织,用免疫组化检测各组小鼠睾丸转化生长因子β1(TGFβ1)、半胱天冬酶3(Caspase-3)的表达;DNA片段原位末段标记技术(TUNEL法)检测睾丸细胞凋亡;单细胞凝胶电泳(彗星实验)检测细胞DNA的损伤。结果染铅组小鼠睾丸TGFβ1、Caspase-3表达增强,凋亡指数增加。与空白对照组相比,差异有统计学意义(P<0.01)。低、中剂量维生素B1、C联合干预后,睾丸细胞DNA损伤降低,凋亡细胞减少,光镜下可见损伤的组织明显改善。与0.2%染铅组比较,差异有统计学意义(P<0.05)。但在高剂量组,维生素联合作用则促进了小鼠睾丸TGFβ1、Caspase-3的表达,使细胞凋亡指数增高,但彗星尾长和拖尾率则显著降低。结论维生素B1、C联合作用可拮抗铅的某些睾丸毒性作用。Objective To investigate the impacts of the combined administration of aseorbie acid and thiamine on certain biochemical and morphologic indexes of testes in mice exposed to lead. Methods 75 male mice were divided into control groups, groups received with 0.2% lead acetate and groups treated by the same lead acetate dose in combination with ascorbic acid and thiamine （subdivided into：low, middle and high-dose） ad libitum with 15 mice in each. 5 mice in each group were sacrificed at 2, 4 and 6 weeks respectively, and then testes were separated from mice. To evaluate the lead toxicity in testis, the levels of TGFβ1 and Caspase-3 were detected by immunohistochemistry, apoptotic cell was determined by terminal deoxynucleotidyl transferase （TdT） mediated dUTP nick end labeling （TUNEL） ; DNA damage of germ cells was assessed by single cell gel electrophoresis （SCGE or Comet assay）. Results The levels of TGFβ1 and Caspase-3, apoptotic index were significantly higher in groups given by lead than those in control groups（ P 〈 0.05 ）. After intervention of low and middle-dose vitamins, DNA damage and the number of apoptotic cell in testis were obviously lower than those of groups exposed to lead（ P 〈 0.05 ）. Also, the impaired tissues were markedly ameliorated under light microscope. Groups administrated with vitamins at the highest dose, however, promoted testicular cell apoptosis via elevated expression of TGFβ1 and Caspase-3, percentage of tail cell and tail length were reduced significantly. Conclusion Thiamine and aseorbie acid could antagonize the action of certain toxicity of testes in mice treated by lead acetate.

关 键 词：铅 睾丸 维生素C 维生素B1 联合作用 

分 类 号：R135.11[医药卫生—劳动卫生] Q56[医药卫生—公共卫生与预防医学]