人芽囊原虫生物学特性——形态特征、繁殖方式及与细菌的关系研究  

Study on the biological characteristic of Blastocystis hominis:morphology,mode of reproduction and the relation to bacteria

作　　者：乔继英[1] 张旭[2] 魏志超[3] 杨珺华[2] 李亚青[3] 张荣[2] 

机构地区：[1]西安交通大学医学院环境与基因相关疾病教育部重点实验室,西安710061 [2]西安交通大学医学院免疫学与病原生物学系 [3]西安交通大学医学院医学检验中心

出　　处：《卫生研究》2006年第6期739-742,共4页Journal of Hygiene Research

基　　金：卫生部科学研究基金资助项目(No.98-1-231)

摘　　要：目的观察人芽囊原虫(Blastocystis hominis,B.h)的生殖方式,对B.h与细菌的关系及相互间微环境的影响进行初步探索。方法分别对新鲜粪便标本和RPMI 1640培养基中B.h,经碘液染色和苏木素染色观察其形态,对B.h阳性的粘液便、粘液血便进行肠道常见致病菌的培养和鉴定,并通过B.h与大肠埃希菌共培养实验,观察两者相互作用。结果本研究确证了四种B.h繁殖方式:二分裂生殖、内二芽生殖、复分裂增殖及出芽生殖。半数B.h阳性标本未检出已知肠道致病菌。B.h对大肠埃希菌有一定抑制作用。结论B.h存在多种繁殖方式;B.h可能单独致病,其致病机制与微生态改变有关。Objective To observe the reproductive modes of Blastocystis hominis and study the relation between this protozoa and bacteria. Methods Using the Iodine and Haematoxylin staining, the morphology of B . h from patients and RPMI 1640 medium were observed. The B. h positive mucous diarrheal specimens were cultured and identified any possible known pathogenic intestinal bacteria. B. h and colibacilltts were co-cultured to observe the interaction between them. Results Four modes of reproduction for B. h were confirmed ： binary fission, endodyogeny, multiple fission and budding. The fact that there was no other intestinal pathogens in half of the B. h positive specimens suggested B. h may cause disease independently. B. h and colibacillus were restrained each other. Conclusion B. h reproducts in at least four modes. B. h could be a pathogen and its pathogenesis may be related to micro-ecological changes.

关 键 词：人芽囊原虫 形态 繁殖方式 细菌 

分 类 号：R531[医药卫生—内科学]