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作 者:张旭[1] 乔继英[2] 答嵘[2] 李亚青[3] 姚繁荣[2]
机构地区:[1]西安交通大学医学院环境与基因相关疾病教育部重点实验室,西安710061 [2]西安交通大学医学院免疫学与病原生物学系 [3]西安交通大学医学院医学检验中心,西安710061
出 处:《卫生研究》2006年第6期743-746,共4页Journal of Hygiene Research
基 金:卫生部科学研究基金资助项目(No.98-1-231)
摘 要:目的建立人芽囊原虫(Blastocystis hominis,B.h)在DMEM单相培养基中的体外连续培养方法,为进一步研究其诊断、生活史、致病机制奠定基础。方法比较不同pH值、血清种类、血清浓度及接种量等条件下虫体生长繁殖情况及影响因素。结果使用DMEM培养基、接种量大于10^5/管、pH7.0~8.0、10%~30%小牛血清(或人、马血清),青、链霉素及二性霉素B,于37℃条件下厌氧培养,每三、六或五天转种一次,可达到体外长期培养B.h的目的。结论使用DMEM单相培养基可用于人芽囊原虫的诊断及体外长期培养。Objective To establish the vitro culture of Blastocystis hominis ( B. h ) in medium DMEM for the further research on diagnosis, life cycle and pathogenicity of this intestinal protoza. Methods The growth, reproduction and relevant factors of B. h under different culture conditions including sorts and concentrations of serum, pHs and number of inoculation were compared. Results Conditions for the continuously anaerobic culture of B. h in medium DMEM were as follows: the number of inoculation were no less than 105 cells per tube, pHs ranged 7.0 - 8.0, concentrations of calf serum (or human serum and horse serum) ranged 10% - 30%, antibiotics and Amphotericin B should be added, subculturing could be choiced at the each peaking-day 3,6 or 5 at 37℃ . Conclusion The medium DMEM could be used in diagnosis and continously vitro culture for B. h.
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