戊型肝炎病毒细胞吸附模型的建立及病毒吸附区域初步研究  

The Establishment of Cellular Attachment Model for Hepatitis E Virus(HEV) and Its Application in the Identification of HEV Cellular Attachment Region

作　　者：何水珍[1] 郑子峥[1] 吴婷[1] 谢明辉[1] 苗季[1] 张军[1] 夏宁邵[1] 

机构地区：[1]厦门大学国家传染病诊断试剂与疫苗工程技术研究中心福建省医学分子病毒学研究中心,福建厦门361005

出　　处：《病毒学报》2006年第6期426-430,共5页Chinese Journal of Virology

基　　金：863计划(2005AA2Z3H20);福建省科技重大专项(2004YZ01);厦门市重大产业科技项目(3502Z20041008)

摘　　要：E.coli中表达的HEV衣壳蛋白片段P239（aa368～606）形成的类病毒颗粒与戊肝患者恢复期血清及中和单抗具有良好的反应性，较好地模拟了天然HEV病毒颗粒的表面空间结构。利用P239吸附HepG2细胞的模型来模拟HEV对宿主细胞的吸附，多株中和单抗对吸附的阻断验证了吸附的特异性。P239与多株传代细胞系的吸附结果则表明了这种特异性吸附的细胞选择性。对阻断P239吸附的线性单抗进行定位，初步确定了P239与细胞相互作用的区域：ORF2上的aa423～443很可能和病毒上的细胞膜受体结合部位非常靠近，或可能直接参与构成了病毒与细胞特异性识别的表位。此本研究为进一步研究HEV与宿主细胞的相互作用提供一定的线索。We have previously expressed a recombinant hepatitis E virus capsid protein p239 （ORF2, aa368-606） which showed good immunogenicity and reactivity, and has been confirmed to have a simulated structure compared with native viruses. In the present study, p239 was used to simulate native HEV to investigate the attachment of HEV on host cells. Our data showed that p239 specifically attached on HepG2 cells as several HEV neutralizing monoclonal antibodies （MAbs） could block p239 attachment on cell surface. Further, by using MAbs against linear epitopes of p239, we identified a possible cellular binding site （aa423-443） for the capsid protein of HEV. Our data provide an important clue for the further analyzing of HEV-host cell interaction.

关 键 词：戊型肝炎病毒(HEV) 类病毒颗粒P239 HEPG2 单抗阻断 

分 类 号：R373.2[医药卫生—病原生物学]