家鸭中H8N4亚型流感病毒株A/duck/Yangzhou/02/2005的全基因克隆和序列分析  

Cloning of the Full-length Genome of H8N4 Subtype Avian Influenza Virus Strain A/duck/Yangzhou/02/2005 and Analysis of the Sequences

作　　者：薛峰[1] 彭宜[1] 张小荣[1] 姚春峰[1] 龙进学[1] 刘秀梵[1] 

机构地区：[1]扬州大学农业部畜禽传染病学重点开放实验室,江苏扬州225009

出　　处：《病毒学报》2006年第6期450-455,共6页Chinese Journal of Virology

基　　金：国家科技攻关项目(2004BA519A02);江苏省属高校重大基础研究项目(05KJA23016)

摘　　要：采用常规的血清学试验和特异性RT—PCR方法对华东地区家养水禽中流感病毒的带毒状况进行4年多的监测，分离鉴定出国内报道的第一株H8亚型禽流感病毒。应用流感病毒通用引物成功地扩增出H8N4亚型禽流感病毒A／duck／Yangzhou／02／2005株（简称Dk／YZ／02／05）的全基因序列。将Dk／YZ／02／05的基因组核苷酸全序列与网上公布的基因序列进行比较分析，结果表明：Dk／YZ／02／05（H8N4）的8个基因片段均含有相应病毒基因的完整开放阅读框；其推导的血凝素（Heamgglutinin，HA）氨基酸剪切位点序列为P-SV-E-P-R，为典型低致病性禽流感病毒的特征序列；神经氨酸酶（NA）在第48位氨基酸后无20个氨基酸的缺失；非结构蛋白（NS）79～84位也没有氨基酸的缺失。An H8N4 subtype avian A influenza virus was isolated from the aquatic birds in some live bird markets when we surveyed the ecology of avian influenza in East China for more than four years and identified by specific RT-PCR. The eight full-length genes, including 5＇ and 3＇ ends of H8N4 subtype avian influenza virus （A/ duck/Yangzhou/02/2005） were amplified by using the universal primers. The amplified segments were cloned into the pGEM-T easy vector, respectively. The results showed that each of the eight gene cDNA fragments contained the whole open reading frame of their own. The NA sequence of Dk/YZ/02/05 was analyzed and the result demonstrated that there had no 20 amino acids missing in 48-68 sites, and no residue lost in NS gene in 263 to 277 sites. The motif of HA cleavage site is P-S-V-E-P-R, which is the characteristics of low pathogenic AIV.

关 键 词：H8N4亚型禽流感病毒 全基因组 序列测定 分析 

分 类 号：S831.7[农业科学—畜牧学]