机械通气对大鼠肺泡巨噬细胞内毒素CD14受体表达的影响  

Effects of mechanical ventilation on the expression of endotoxin receptor CD14 in alveolar macrophages in rats

作　　者：李克忠[1] 姚尚龙[2] 孙国勇[1] 潘恩木[1] 冯颢[1] 马利[2] 

机构地区：[1]山东大学第二医院麻醉科,山东济南250033 [2]华中科技大学同济医学院协和医院麻醉科,湖北武汉430022

出　　处：《山东大学学报（医学版）》2006年第11期1142-1145,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助课题(30571787);山东省自然科学基金资助课题(Y2006C120)

摘　　要：目的:探讨机械通气对大鼠肺泡巨噬细胞(alveolar macrophages,AM)内毒素受体CD14表达的影响。方法:清洁级成年雄性SD大鼠18只,随机分为自主呼吸组(A组)、小潮气量机械通气组(S组)和大潮气量机械通气组(L组)3组,每组各6只。实验4 h结束,放血处死大鼠。测定大鼠肺病理形态学积分、肺组织湿/干重比(W/D)、支气管灌洗液(BALF)炎性细胞数和肺蛋白透性系数。RT-PCR测AM上内毒素受体CD14的表达,免疫组化法测BALF里AM上的CD14表达。结果:与A组比较,L组肺病理形态学积分、W/D、WBC和肺蛋白透性系数、BALF里AM的CD14蛋白表达和CD14基因表达显著升高(P<0.01),S组改变差异无统计学意义(P>0.05)。结论:大潮气量机械通气导致大鼠的肺部损伤,并使AM上CD14表达明显上调。小潮气量机械通气可避免上述改变的发生。Objective： To investigate the effects of mechanical ventilation on endotoxin receptor CD14 in alveolar macrophages in rats. Methods： Eighteen clean adult male SD rats were respectively dealt with autonomous breathing （A group, n = 6）, small tidal volume mechanical ventilation （ S group, n = 6） and large tidal volume mechanical ventilation （L group, n = 6）at random. Four hours later, the experiment was terminated and the rats were executed by bloodletting. Then the pulmonary pathomorphology integral, the pulmonary tissue wet/dry weight ratio, WBC in bronchial lavage fluid（BALF）, and the pulmonary albumin permeability （PAP）were determined. The expression of AM endotoxin receptor CD14 mRNA was determined by reverse transcription-polymerase chain reaction （RT-PCR）, of AM CD14 protein by immunohistochemistry. Result： Compared with A group, pulmonary pathomorphology integral, W/D ratio, WBC, PAP, AM CD14 protein expression, and AM CD14 mRNA expression were not significantly changed in the S group（ P 〉 0.05） ;but the above values were significantly increased in the L group（ P 〈 0.01 ）. Conclusion： Large tidal volume mechanical ventilation can induce the injury of lungs and up-regulate the CD14 expression in alveolar macrophages in rats. Small tidal volume mechanical ventilation can avoide the above-mentioned changes.

关 键 词：机械通气 机械感受器 内毒素类 肺泡巨噬细胞 

分 类 号：R614.2[医药卫生—麻醉学]