表达γ-亚麻酸的无筛选标记转基因大豆的鉴定  

Characterization of Marker-free Transgenic Soybeans with Δ~6-fatty Acid Desaturase Gene Expressing γ-linolenic Acid

作　　者：张秀春[1] 林俊扬[2] 彭明[1] 郭丽琼[1] 林俊芳[1] 

机构地区：[1]中国热带农业科学院热带作物生物技术研究所,海口571101 [2]福建省莆田市农业科学研究所,福建莆田351100

出　　处：《热带亚热带植物学报》2006年第6期460-466,共7页Journal of Tropical and Subtropical Botany

基　　金：广东省科技攻关项目(2005B20101009)资助

摘　　要：对所获得的9株无筛选标记基因的T1代莆豆8008转基因大豆的T2代进行了分析。PCR和Southern杂交检测表明标记基因已不存在于转基因株系中,同时目的基因以纯合或者杂合状态存在于部分株系中,除草剂抗性分析也表明这些转基因株系已不再具有抗除草剂的能力。Northern杂交检测表明Δ6-脂肪酸脱氢酶基因在转基因株系中可以转录。GC分析表明其中6个转基因植株富含GLA。Nine lines of T2 generation of marker-flee transgenic soybeans of Pudou 8008 with △^6-fatty acid desaturase gene were characterized. PCR and Southern blot indicated that the selective marker gene （bar） had been removed from some transgenic soybean progenies, while △^6-fatty acid desaturase gene was still in some progenies of these transgenic soybean lines. Northern blot showed that the △^6-fatty acid desaturase gene could be transcribed in the marker-free transgenic soybean lines. Gas chromatographic analysis showed that six transgenic soybean lines could produce γ-linolenic acid.

关 键 词：大豆 △^6-脂肪酸脱氢酶基因 无标记基因 Γ-亚麻酸 SOUTHERN BLOT NORTHERN BLOT GC 

分 类 号：S565.1[农业科学—作物学]