不同剂量维生素E对去甲肾上腺素诱导心肌细胞凋亡的影响  

Effect of Vitamin E at different dose on Norepinephrine-induced apoptosis in cardiac myocytes

作　　者：吴强[1] 王晓峰[1] 蔡运昌[1] 杨天和[1] 陈吉[2] 

机构地区：[1]贵州省人民医院心内科,贵阳550001 [2]中国人民解放军第44医院心内科,贵阳550004

出　　处：《中国分子心脏病学杂志》2003年第2期90-93,共4页Molecular Cardiology of China

基　　金：贵州省科学技术基金(No.3044)

摘　　要：目的 探讨维生素E对去甲肾上腺素(NE)诱导的心肌细胞凋亡的影响及其机制。方法:培养乳鼠心肌细胞暴露于NE(20umol/L)和维生素E(5μmol/L、50μmol/L及500μmol/L)36小时,检测心肌细胞凋亡率和DNA梯状带纹的变化。结果:NE可导致心叽细胞凋亡率明显增高和DNA梯状带纹出现,500μmol/L的维生素E干预可抑制NE的致心肌细胞凋亡作用。结论:NE的促凋亡作用可能与活性氧生成增加有关,维生素E以剂量依赖方式抑制NE的促凋亡作用。Objective To study the effects and mechanisms of vitamin E on norepinephrine （NE）-induced apoptosis in cardiac myocytes. Methods After exposed to NE alone （20μmol/L）and NE ＋ vitamin E （5μmol/L,50μmol/L and 500μmol/L）for 36 hours, the percentage of apoptotic myocytes and DNA laddering were investigated in cardiomyocytes. Results NE increased the percentage of cells that were stained by TUNEL and DNA laddering on agarose gel electrophoresis. Pretreatment with Vitamin E at 500μmol/L inhibited NE-induced myocardial cell apoptosis. Conclusion NE-induced cardiomyocytes apoptosis is associated with overproduction of reactive oxygen species. Vitamin E inhibits the effect of NE in dose-dependence way.

关 键 词：维生素E 去甲肾上腺素 凋亡 心肌细胞 

分 类 号：R541[医药卫生—心血管疾病]