RP-HPLC法测定更舒安颗粒中淫羊藿苷  

Determination of Icariin in Gengshu’an Granules by RP-HPLC

作　　者：陈卫东[1] 黄轶君[1] 葛秀文[1] 吴萍[1] 惠永正[1] 王骏[2] 徐宏喜 

机构地区：[1]上海中药创新研究中心,上海201203 [2]香港中文大学 [3]香港赛马会中药研究院

出　　处：《国外医药（植物药分册）》2006年第6期251-253,共3页

基　　金：香港赛马会慈善基金

摘　　要：目的:为控制新药更舒安颗粒的产品质量,建立其有效成分淫羊藿苷的定量方法。方法:淫羊藿苷的定量测定采用RP-HPLC法。色谱柱为ZORBAXSBC18(250mm×4.6mm,5μm),流动相为乙腈-水(30∶70),流速1.00mL/min;柱温为25℃;检测波长为270nm。结果:淫羊藿苷在测定条件下,平均回收率为101.18%,RSD=0.62%,回归方程为Y=20.121X-3.7077,r=1.0000;最低检出限度为0.12μg/mL,线性范围在1.22-24.48μg/mL;精密度试验淫羊藿苷峰面积RSD为0.58%。结论:本方法准确、简便、专属性强、灵敏度高,结果稳定,适用于更舒安颗粒的质量控制。Objective To establish RP-HPLC method for the determination of icariin in Gengshu＇ an Granules. Methods The Column used was ZORBAX SB C18（250 mm×4.6 mm, 5μm）, the mobile phase used was acetonitrile water （30 ： 70）, the flow rate was 1.00 mL/min with the detection wavelength at 270 nm. Results The minimum detection limit is 0.12μg/mL, the average recovery was 101.18% with RSD 0.62%, the calibration curve was the linear in the range of 1.22 24.48μg/mL, r=1.0000. Conclusion The method is accurate, simple, specific, sensitive, and result is stable. It can be used for the quality control of Gengshur an granules.

关 键 词：淫羊藿苷 RP-HPLC 更舒安颗粒 质量控制 

分 类 号：R286.0[医药卫生—中药学]