水稻(Oryza sativa L.)苗期低温白化突变体al12的超微结构与基因定位(英文)  

Ultrastructure and Gene Mapping of the Albino Mutant al12 in Rice (Oryza sativa L.)

作　　者：夏九成[1] 王玉平[1] 马炳田[1] 殷兆晴[1] 郝铭[1] 孔德伟[1] 李仕贵[1] 

机构地区：[1]四川农业大学水稻研究所

出　　处：《Acta Genetica Sinica》2006年第12期1112-1119,共8页

基　　金：This work was supported by Program for New Century Excellent Talents in University (No. NCET-04-0907) and Program for In-novative Research Team in University (No.IRT0453).

摘　　要：水稻苗期低温白化突变是水稻在发育早期对低温胁迫的一种适应性,是一种受发育和温度控制的条件表达,它与其他水稻白化突变有本质的不同。本研究利用便携式叶绿素测量仪测定了白化时期植株的叶绿素含量和用透射电镜观察了叶绿体的结构变化。结果发现叶绿素平均含量仅为1.2(SPAD),而叶绿体也不能正常发育仅有囊泡状结构。通过与9311的正反交实验及子代的分离表现证明该性状受一个隐性核基因的控制。另外利用SSR分子标记技术将该基因定位在第8染色体上,两侧最近的SSR标记RM5068和RM3702分别距基因0.5～1.1cM和4.9cM,基因被定位在约6个cM的区间内。我们将该基因暂时命名为al12。Seedling albino mutation resistant to low temperature is an adaptability of rice （Oryza sativa L.） to cold. The mutant, a conditional expression controlled by development and temperature, differs from other albino mutants. The chlorophyll content of the mutant was measured using a portable chlorophyll meter, and the ultrastructure of the chloroplast was observed using a transmission electron microscope. Chlorophyll content was 1.2 SPAD, and the chloroplast did not develop, with only small vesicle-like structures. A segregation analysis of the reciprocal crosses between the albino mutation line with the rice line 9311 demonstrated that the albino trait was controlled by a single recessive gene, which was flanked by SSR markers RM5068 and RM3702 on the short arm of chromosome 8 with a distance of 0.5-1.1 cM and 4.9 cM, respectively. This gene was mapped within a 6 cM interval region and was tentatively referred to as all2.

关 键 词：水稻 白化突变体 叶绿体 基因定位 

分 类 号：S511[农业科学—作物学]