栝楼的组培快繁技术研究  

Tissue Culture and Rapid Propagation of Trichosanthes kirilowii

作　　者：杨晓伶[1] 金关荣[2] 杨端鹏[1] 李珊[1] 朱云国[1] 

机构地区：[1]同济大学生命科学与技术学院,上海200092 [2]浙江省农科院花卉研究开发中心,浙江杭州311202

出　　处：《中药材》2006年第11期1129-1130,共2页Journal of Chinese Medicinal Materials

摘　　要：目的：探讨栝楼组织培养及种苗快速繁殖技术。方法：通过茎尖、带芽茎段、茎段和叶片诱导丛生芽及愈伤组织。结果：栝楼2年生块茎幼苗的茎尖和带芽茎段在MS＋2mg／LBA＋0．5～0．05mg／LNAA培养基上可诱导丛生芽，并在生根培养基MS＋0．1mg／LNAA＋0．2mg／LIBA中生根，经驯化移栽可实现快速繁殖。无芽茎段和叶片用含4mg／LBA和0．5mg／LNAA的MS培养基可诱导愈伤组织，由茎段来的愈伤组织经6周后分化为无根苗。结论：栝楼茎尖和带芽茎段的组织培养，可在短期内大量繁殖栝楼雌雄种苗，具有成本低，效益高的特点。Objective： To study tissue culture of Trichosanthes kirilowii, and establish the technique of rapid propagation. Methods： Cluster buds were induced from stem tip and stem with axillary bud, calluses were induced from stems and leaves. Results： Cluster buds could be induced on both the bud and axillary bud from 2-years-old tuber of Trichosanthes kirilowii with MS medium containing 2 mg/L BA and 0. 5 - 0. 05 mg/L NAA. The roots could be induced with MS ＋ 0. 1 mg/L NAA ＋ 0. 2 mg/L IBA. The seedling with roots could be transplanted after domestication, and achieved the rapid seedling propagation. Calluses could be induced from the stems and leaves with MS ＋4 mg/L BA ＋0. 5 mg/L NAA, and the calluses then could be differentiated into seedlings without root. Conclusion： The male and female seedlings of Trichosanthes kirilowii can be propagated largely using stem tip or axillary bud in short period. The technique of rapid propagation on Trichosanthes kirilowii have a high benefit and low costs.

关 键 词：栝楼 组培 快速无性繁殖 

分 类 号：R282.2[医药卫生—中药学]