从下丘脑单胺类神经递质含量的四季变化探索“肾通于冬气”的机制  

Study on the Seasonal Changes of Monoamine Neurotransmitter in Hypothalamus to Probe into the Mechanism of the Kidney Responding to Winter

作　　者：罗卫芳[1] 张家俊[2] 郭霞珍[2] 郭树仁[3] 苏晶[2] 金光亮[2] 

机构地区：[1]中国中医科学院中医基础理论研究所,北京100700 [2]北京中医药大学,北京100029 [3]北大维信生物科技有限公司,北京100080

出　　处：《中国中医基础医学杂志》2006年第11期815-817,共3页JOURNAL OF BASIC CHINESE MEDICINE

摘　　要：目的:观察雄性金黄地鼠下丘脑单胺类神经递质的四季变化,探索肾主生殖“通于冬气”的机制。方法:分别于春分、夏至、秋分、冬至分离金黄地鼠下丘脑组织,采用高效液相色谱-电化学法检测下丘脑NE、DA、HVA、5-HT、5-HIAA等的含量。结果:下丘脑NE含量在冬、春季较低,夏、秋季较高,与性腺功能的四季变化趋势相似;下丘脑5-HT及其代谢产物5-HIAA含量则显示为冬、春季明显高于夏、秋季的变化趋势,与性腺功能的四季变化趋势相反。结论:在性腺功能随季节变化的过程中,下丘脑NE可能起着调节作用,下丘脑5-HT可能主要起抑制作用。Objective： To probe into the mechanism of kidney responding to winter （seasonal changes reproduction） by investigating the seasonal changes of monoamine neurotransmitter and their metabolites golden hamster hypothalamus Methods： To measure norepinephrine （ NE ）, dopamine （ DA ）, in in 5- hydroxytryptamine （5-HT）, 5-hydroxy-3-indoleacetic acid （5-HIAA）, homovanillic acid （HVA） in golden hamster hypothalamus gotten on Spring Equinox, Summer Solstice, Autumnal Equinox and Winter Solstice by high performance liquid chromatography with electrochemical detector. Results：The level of NE was lower in winter and spring than in summer and autumn, and showed the same tendency with the seasonal changes of reproduction.The level of 5-HT and 5-HIAA was higher in winter and spring than in summer and autumn, which showed a contrary tendency with the seasonal changes of reproduction. Conclusion ： It is possible that NE in hypothalamus adjusts or controls the seasonal changes of reproduction, and 5-HT plays inhibiting action in seasonal changes of reoroduction.

关 键 词：肾主生殖 季节 下丘脑 单胺类神经递质 高效液相色谱 

分 类 号：R2-031[医药卫生—中西医结合]