表皮生长因子受体在紫外线诱导血管内皮生长因子分泌中的调控  

The role of EGFR on UV-induced VEGF secretion of MEF

作　　者：李燕华[1] 毕志刚[1] 

机构地区：[1]南京医科大学第一附属医院皮肤科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2006年第12期1159-1162,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(30671894);江苏省重点学科基金(135-03);江苏省研究生创新基金资助

摘　　要：目的:探讨表皮生长因子受体(EGFR)在紫外线诱导血管内皮生长因子(VEGF)分泌中的作用。方法:流式细胞仪检测EGFR和磷酸化EGFR的表达。ELISA检测上清中VEGF的水平。结果:紫外线照射后15min可以检测到磷酸化EGFR的表达,30min时达最高峰,2～8h降至基础水平。在整个过程中EGFR的表达量不变。(EGFR+\+)MEF细胞组VEGF分泌明显高于(EGFR+/-)MEF和(EGFR-/-)MEF细胞组,(EGFR-/-)MEF细胞组中波紫外线对VEGF分泌促进作用不明显。PD153035,可明显抑制VEGF分泌,1!m有抑制作用,5!m抑制作用增强。结论:EGFR是UVB诱导VEGF分泌信号途径中的关键靶点。Objectives： To explore the role of epidermal growth factor receptor （EGFR） on UV-induced VEGF secretion of MEF. Methods： Flow cytometry was used to detect total receptor and tyrosine-phosphorylated EGFR, respectively, the concentration of VEGF was determined by ELISA. Results： EGFR phosphorylation was detectable within 15 min after UV exposure. The level of EGFR phosphorylation reached peak 30 min after UV exposure, and was decreased to basal levels 2 to 8 h after UV exposure. VEGF expression was not increased in EGFR （-/-） MEF following UV exposure. In contrast VEGF expression, was significantly increased in egfr （＋/＋） MEF after UV exposure. PDI53035 inhibited the induction of VEGF protein expression in UVB-treated cells in a dosedependent manner. Conclusion： EGFR plays an important role in UV-induced VEGF secretion in MEF.

关 键 词：紫外线 表皮生长因子受体 血管内皮生长因子 

分 类 号：R392.11[医药卫生—免疫学]