青心酮对妊高征患者胎盘血管壁一氧化氮合成酶和血浆内皮素的影响  

TheEffectsof3,4┐DihydroxyacetophynoneontheActivityofNitricOxideSynthetaseinPlacentalVascularEndothetialCelsandSmoothMuscleCelsandontheLevelofEndothelin┐1inPlasmafromPregnancy┐InducedHypertensionPatients

作　　者：黄引平[1] 叶笃筠[1] 马庭元[1] 吴熙瑞[1] 陈汉平[1] 邱爱芝 李静[1] 罗茂兰 

机构地区：[1]同济医科大学附属同济医院妇产科

出　　处：《中华妇产科杂志》1996年第11期667-669,共3页Chinese Journal of Obstetrics and Gynecology

基　　金：国家自然科学基金

摘　　要：目的：观察青心酮（３，４二羟基苯乙酮，ＤＨＡＰ）对妊高征（ＰＩＨ）患者胎盘血管内皮细胞（ＶＥＣ）和平滑肌细胞（ＶＳＭＣ）内一氧化氮合成酶（ＮＯＳ）活性和血浆内皮素（ＥＴ）水平的影响。方法：将２２例ＰＩＨ患者随机分为妊高征组和ＤＨＡＰ治疗组（ＤＨＡＰ每天１６０～２４０ｍｇ），同时取１０例非妊娠妇女（对照组）及１０例正常妊娠妇女（正常妊娠组）作对照。于给药前或（和）剖宫产前采外周静脉血，分离血浆，采用放射免疫法测定ＥＴ；取新鲜胎盘行组织化学分析。结果：正常妊娠组胎盘ＶＥＣ和ＶＳＭＣ内ＮＯＳ活性较高，而妊高征组相应细胞内ＮＯＳ活性明显减弱，并伴有组织和细胞的形态学损伤；ＤＨＡＰ治疗组ＮＯＳ活性较治疗前或未经ＤＨＡＰ治疗的ＰＩＨ患者明显增加，其组织和细胞损伤也减轻；正常妊娠组血浆ＥＴ水平高于对照组而妊高征组血浆ＥＴ水平又高于正常妊娠组，ＤＨＡＰ治疗组血浆ＥＴ水平明显低于妊高征组。结论：ＤＨＡＰ可纠正病理性一氧化氮／内皮素失衡，对改善孕妇及胎儿血液循环有重要作用。Objective:Toobservetheefectsof3,4-dihydroxyacetophynone(DHAP),oneoftheconstituentsofatraditionalChineseherbalmedicine,ontheactivitiesofendothelialnitricoxidesyn-thetase(NOS)invascularendothelialcels(VEC)andvascularsmoothmusclecels(VSMC)ofplacentaandthelevelofendothelin-1(ET-1)intheplasmafromPIHpatients.Method:22nuli-parousPIHpatientswererandomlydividedintoPIHgroupandDHAPtreatmeatgroup.Eachpa-tientsinDHAPgroupreceivedintravenousadministrationofDHAP(160～240mg/d).BloodsampleswerecolectedbeforetheadministrationofDHAPand(or)beforecesareansection.Theplacentawereassayedfornitricoxidesynthaseactivityusinghistochemicalanalysis;theconcentrationofETinplasmawasmeasuredbyradioimmunoassays.10normalpregnantwomenor10nonpregnantwom-enwereservedascontrols.Results:Innormalpregnantcontrols,NOSactivitywasmuchhigherthanthatinthePIHgroup;inDHAPgroup,therewassomerecoveryofNOSactivityafterDHAPtreatment.TheconcentrationofETwashigherinPIHgroupthanthatofnormalpregnantcontrols,butitdecreasedsignificantlyafterDHAPtherapy(P<0.05,vsPIHgrouporbeforeDHAPtherapy).Conclusion:ThisstudyindicatesthatDHAPiseffectiveinthetreatmentofPIHanditsmechanismofactionmaybeduetotheadjustmentofNO/ETimbalanceinPIHpatients.

关 键 词：妊娠高血压 胎盘 一氧化氮 内皮素 青心酮 

分 类 号：R714.25[医药卫生—妇产科学]