实时荧光聚合酶链反应检测呼吸道感染鲍曼不动杆菌TEM-1基因  

Detection of TEM-1 β-lactamase gene of Acinetobacter baumannii isolated from sputum by real-time PCR

作　　者：张红河[1] 董晓勤[1] 范建中[1] 周田美[1] 王贤军[1] 

机构地区：[1]杭州市第一人民医院基因诊断实验室,浙江杭州310006

出　　处：《中国微生态学杂志》2006年第6期466-467,共2页Chinese Journal of Microecology

基　　金：杭州市医药卫生科技项目(2003A007)

摘　　要：目的建立一种快速、准确、特异、定时检测鲍曼不动杆菌TEM-1型β-内酰胺酶耐药基因的方法。方法选择TEM-1型β-内酰胺酶耐药基因作为靶序列,设计合成引物和探针;收集呼吸道感染患者痰标本培养的鲍曼不动杆菌279株,并对其进行检测分析。结果采用荧光聚合酶链反应检测鳗曼不动杆菌TEM-1耐药基因灵敏度为102拷贝,279株鲍曼不动杆菌中检出47株携带TEM-1基因,检出率为16.8%。结论应用Taqman探针荧光聚合酶链反应能够快速、准确检测鲍曼不动杆菌TEM-1型β-内酰胺酶耐药基因。Objective To develop a real-time fluorescence PCR assay to detect the TEM-1 β-lactamase gene of Acinetobacter baumannii. Methods To design and synthesize the primers and TaqMan probe of the TEM-1 β-lactamase gene,279 isolates of Acinetobacter baurnannii originating from sputum samples were analyzed. Results The sensitivity of the assay for he TEM-1 β-lactamase gene was 10^2 copies. Among 279 samples,47 strains were positive for TEM-1 β-lactamase gene （16.8% ,47/279）. Conclusion The TaqMan PCR could rapidly and exactly detect the TEM-1 β-lactamase gene of Acinetobacter baumannii

关 键 词：聚合酶链反应 鲍曼不动杆菌 TEM-1基因 

分 类 号：R378.99[医药卫生—病原生物学]