HPLC-UV与HPLC-ELSD测定环丙沙星含量之比较  

The comparison of determination of ciprofloxacin by HPLC-UV and HPLC-ELSD

作　　者：乔成孝[1] 裴朝玉[2] 陈晓刚[3] 方国波[1] 

机构地区：[1]南开大学生命科学学院,天津300071 [2]周口师范学院化学系,河南周口市466000 [3]天津市琛航科技有限公司,天津300070

出　　处：《现代仪器》2006年第6期29-31,共3页Modern Instruments

摘　　要：目的:探讨利用HPLC-紫外检测器(UV)和HPLC-蒸发光散射检测器(Evapo-rative light scattering detector,ELSD)测定乳酸环丙沙星氯化钠注射液中环丙沙星含量,比较两种检测方法的优劣。方法:色谱条件为:色谱柱:Kromasil C-18,5,μ250×4.6mm;流动相:三氟乙酸∶乙腈=80∶20(V/V);流速:1mL/m in;柱温:25℃;检测波长:277nm;蒸发光散射检测器条件:雾化温度30℃,漂移管温度65℃。结果:环丙沙星注射液中的各种成分在上述色谱条件下能完全分离,分析时间仅需20m in;两种测定方法结果一致。结论:紫外检测器更加灵敏和稳定,而蒸发光散射检测器则适用于更多的物质。Objective： To approach an assay of determination of ciprofloxacin in Ciprofloxacin Lactatc and Sodium Chloride Injection by HPLC - UV and HPLC - ELSD methods. Make a comparison between the two different methods. Methods： The separation was performed on a Kromasil C - 18 column （5 μ,250 × 4.6mm）. The; n,ohile phase consists of trifluoroacetie acid（0. 1% ） -acetonitrile（80： 20） at a flow rate of 1.0 mL/min. Determination wavelength： 277mn. An evaporative light -scattering detector （ELSD） Modcl 200（SoftA, USA） was used, its para,neters were set as follows： spray temperature at 30℃, drift tube temperature at 65℃. Results： Good separation of Ciprofloxacin and related substances can be achieved respectively under the two chromatographic system only in 20 minutes. The two methods could give the same determination. Conclusion： The UV detection is more sensitive and more steady, and ELSD detection could be used in determination of more substances.

关 键 词：高效液相色谱法 紫外检测器 蒸发光散射检测器 环丙沙星 

分 类 号：TQ465[化学工程—制药化工]