多巴胺在dsDNA/Ni^(2+)/聚邻氨基酚修饰碳糊电极上的伏安行为  

Voltammetric Behavior of Dopamine at dsDNA-modified Ni^(2+)/poly(o-aminophenol)Carbon Paste Electrode

作　　者：杨涛[1] 焦奎[1] 杨婕[1] 赵常志[1] 曲文营[1] 

机构地区：[1]青岛科技大学化学与分子工程学院

出　　处：《高等学校化学学报》2006年第12期2294-2296,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20375020);青岛市自然科学基金(批准号:04-2-JZP-8)资助.

摘　　要：The Ni 2+/poly（o-aminophenol）electropolymerization was carried out by a continuous cyclic voltammetric scanning at a carbon paste electrode in a mixture of 0.02 mol/L o-aminophenol,0.3 mol/L NiSO 4 and 0.6 mol/L HClO 4.dsDNA was linked to the conducting polymer via three-dimensional structure of polymer film and electrostatic attraction between the negatively charged phosphate backbone of DNA and Ni 2+ ion.The results were testified by the electrochemical methods by using Co（phen）2+3 as an electrochemical indicator.The currents of redox peaks of Co（phen）2+3 increased after immobilizing dsDNA.The conditions for the preparation of Ni 2+/P-OAP/CPE and DNA immobilization were optimized.The voltammetric behavior of dopamine was investigated at the modified electrode.It was found that the modified electrode exhibited a highly electrocatalytic activity toward dopamine oxidation.Differential pulse voltammetry was used for determination of dopamine in pH 5.1 phosphate buffer solution.A linear response of the peak current versus the concentration was found in the range from 5.0×10-6 to 2.0×10-4mol/L with 1.0×10-2 mol/L AA（ascorbic acid）coexisting.The presence of a high concentration of AA did not interfere with the determination.The proposed method exhibits a good recovery and reproducibility.The Ni^2＋/poly （o-aminophenol） electropolymerization was carried out by a continuous cyclic voltammetric scanning at a carbon paste electrode in a mixture of 0. 02 mol/L o-aminophenol, 0. 3 mol/L NiSO4 and 0. 6 mol/L HCIO4. dsDNA was linked to the conducting polymer via three-dimensional structure of polymer film and electrostatic attraction between the negatively charged phosphate backbone of DNA and Ni^2＋ ion. The results were testified by the electrochemical methods by using Co（phen）3^2＋ as an electrochemical indicator. The currents of redox peaks of Co（phen）3^2＋ increased after immobilizing dsDNA. The conditions for the preparation of Ni^2＋/P-OAP/CPE and DNA immobilization were optimized. The vohammetric behavior of dopa- mine was investigated at the modified electrode. It was found that the modified electrode exhibited a highly electrocatalytic activity toward dopamine oxidation. Differential pulse vohammetry was used for determination of dopamine in pH 5. 1 phosphate buffer solution. A linear response of the peak current versus the concentration was found in the range from 5.0×10^-6 to 2. 0×10^-4mol/L with 1. 0× 10^-2mol/L AA（ascorbic acid） coexisting. The presence of a high concentration of AA did not interfere with the determination. The proposed method exhibits a good recovery and reproducibility.

关 键 词：邻氨基酚 NI^2+ DNA 多巴胺 抗坏血酸 

分 类 号：O657.1[理学—分析化学]