高效液相色谱法测定蛇床子萃取物中的蛇床子素  

Using HPLC to determine osthole in extracts from fructus cnidii obtained by supercritical CO_2 extraction and traditional ethanol extraction

作　　者：张晓霞[1] 周卯星[1] 张高勇[1] 王佩维[1] 宫竹云[1] 

机构地区：[1]中国日用化学工业研究院,山西太原030001

出　　处：《日用化学工业》2006年第6期395-397,共3页China Surfactant Detergent & Cosmetics

基　　金：山西省高级专家资助项目(204512)

摘　　要：采用高效液相色谱(HPLC)法对传统乙醇提取法(SE)与超临界CO2流体萃取法(SFE-CO2)所得的蛇床子萃取物中所含蛇床子素进行分析测定和比较。首先用柱层析对样品进行预处理,然后用HPLC外标法测定。色谱条件:Diamonsil C18柱,V(甲醇)∶V(水)=80∶20为流动相,检测波长322 nm,柱温35℃。结果表明蛇床子素在6.24μg/mL^37.44μg/mL,峰面积与浓度呈良好的线性关系,相关系数r=0.999 9,方法精密度RSD=1.46%,回收率99.09%~101.86%;SFE-CO2萃取物中蛇床子素的质量分数比SE提高了约11%。The HPLC method for determination of osthole was established. The extracts from fructus cnidii were obtained by supercritical CO2 extraction （SFE- CO2） and ethanol extraction （SE）. The analysis of osthole in these different extracts were studied in comparison by HPLC. Column chromatography with silica- gel as the stationary phase was used firstly for pretreatment and external standard HPLC was used for quantitative analysis. The column is Diamonsil Cls. The mobile phase consists of methanol- water （80：20）. The detective wavelength is 322 nm with the column temperature of 35 ℃. The results showed that the linear range of the standard curve is 6.24μg/mL ～ 37.44 μg/mL （ r = 0.999 9）. The recovery is between 99.09% and 101.86%, and RSD is 1.46%. The content of osthole in extract obtained by SFE- CO2 increases by 11% than that by SE.

关 键 词：化妆品添加剂 蛇床子素 高效液相色谱法 测定 

分 类 号：TQ658[化学工程—精细化工]