克雷伯杆菌利用生物柴油副产物甘油生产氢气和1,3-丙二醇的研究  

Hydrogen and 1,3-Propanediol Production from Glycerol in Biodiesel Wastes by Klebsiella pneumoniae

作　　者：刘飞[1] 方柏山[1] 

机构地区：[1]华侨大学工业生物技术福建省高等学校重点实验室

出　　处：《食品与发酵工业》2006年第10期1-4,共4页Food and Fermentation Industries

基　　金：国家自然科学基金资助项目(20276026;20446004);福建省科技计划项目重点项目(2003I020)

摘　　要：以Klebsiella pneumoniae DSM2026为出发菌株，通过紫外线诱变，选育得到能耐较高浓度生物柴油副产物甘油生产H2和1，3-丙二醇（1，3-PD）的菌株21株，命名为Kp1～Kp21。通过比较，Kp8菌株产量最高，1，3-PD和H2产量分别达到0．36g/50mL和0．99mmol/50mL，比出发菌株分别提高了3．5倍和4．2倍。对Kp8菌株发酵条件进行优化，得到最佳培养条件为pH7．0，培养温度37℃，接种量10％（v/v），废甘油浓度为30g／L。在该条件下H2产量为1．0mmoL/50mL，1，3-PD产量为7．5g/L，甘油转化率为83．3％。Klebsiella pneumoniae DSM2026 was used as the original strain to breed the mutant strains that were able to utilized high concentration of biodiesel wastes containing glycerol to produce hydrogen and 1,3 - propanediol（1,3-PD）. High- yield mutant strains named Kp1- Kp21 were obtained by UV rays treatment, and the mutant strain Kp8 got the highest production, attaining 0.36g/50mL （1,3-PD） and 0.99mmol/50mL （hydrogen）, 3.5 and 4.2 times higher than original strain, respectively. After culture condition was optimized, the optimal initial pH, temperature, inoculums and waste glycerol concentration were 7.0, 37℃, 10 96 （v/v） and 30 g/L, respectively. In this condition the hydrogen and 1,3-PD production were 1.0mmol/50mL and 7.5g/L, respectively, and the conversion rate of glycerol was 83.3 %.

关 键 词：克雷伯杆菌 生物柴油 氢气 1 3-丙二醇 紫外诱变 筛选 

分 类 号：TQ517.43[化学工程]