用4对引物聚合酶链反应检验鼠疫菌的研究  

Study on the Test of Examining Yersinia pestis by 4-pair-primer-PCR

作　　者：杜国义[1] 史献明[1] 刘合智[1] 张月枝[1] 白晓英[1] 李玉贵[1] 白万翔[1] 王桂琴[1] 白雪微[1] 王海峰[1] 杨顺林[1] 胡乐乐[1] 

机构地区：[1]河北省鼠疫防治所检验科,张家口075000

出　　处：《中国媒介生物学及控制杂志》2006年第6期473-475,共3页Chinese Journal of Vector Biology and Control

摘　　要：目的以传统的“四步检验”法为“金标准”,用4对引物聚合酶链反应(PCR)检测鼠疫动物材料,从而探索一种鼠疫快速诊断的方法。方法毒力测定和4对引物PCR按常规方法进行。结果在150份动物材料中,传统的“四步检验”作为金标准共检出阳性62份,而PCR法共检出阳性52份,经χ2检验,差异无统计学意义(χ2=1.42,P>0.05)。PCR实验在4h内全部完成。结论4对引物PCR法具有快速、敏感等优点,可以用于鼠疫的快速诊断和流行病学调查。Objective Plague animal material is tested by 4-pair primer PCR which is eompaired with traditional ＂4 piece test＂ to find a quick diagnostic way for plague. Methods Narcotics test and 4-pair-primer PCR are both done in traditional way. Results 52 positive specimen of 4-pair-primer-PCR are found from 150, and 62 of traditional way are found. Both the ways have no discrepancy （χ^2 = 1.42, P 〉 0.05）. Conclusion 4-pair-primer-PCR is a quick, peculiar, sensitive method for the diagnosis and surveillance of plague.

关 键 词：4对引物 聚合酶链反应 鼠疫菌 

分 类 号：R378.61[医药卫生—病原生物学]