HBV基因型及其临床意义的研究  

Study on hepatitis B virus genotype and its clinical significance

作　　者：杨爱平[1] 崔荣霞 唐剑武 钱开成 邰立慧 王定祥 

机构地区：[1]南通大学附属海安医院感染科,江苏省226600

出　　处：《实用诊断与治疗杂志》2006年第12期861-862,865,共3页Journal of Practical Diagnosis and Therapy

基　　金：南通市科技局科研课题(2005S5852)

摘　　要：目的:探讨乙型肝炎病毒基因型与临床表现及干扰素联合猪苓多糖抗病毒治疗后HBV DNA、HB eA g的关系。方法:研究病例39例,入选病例32例,采用微板核酸杂交-EL ISA法进行基因分型,干扰素a-2b 500万u,每周3次,疗程6个月;同时给予猪苓多糖4 mL,1次/d,疗程2个月,观察HBV DNA、HB eA g、HB eA g/抗-HB e的变化。结果:32例样本中,B型,C型,B、C混合型分别为37.5%、50%、12.5%,发现家族中连续二代基因型的变化,同时发现联合抗病毒治疗的疗效并未在基因亚型中产生差异。结论:慢性HBV感染者不同类型肝病中基因型B和基因型C所占比例有差异。从家族中连续二代基因型的变化可以看出,基因分型又是相对的,基因亚型间存在自然的变异。Objective To investigate the relationships between hepatitis B virus genotypes and clinical manifestations, and effect of interferon and Zhuling polysaccharide therapy on chronic hepatitis B. Methods HBV genotypes of 32 patients were selected with PCR microplate sandwich hybrization-ELISA technique. All were treated with interferon of a-2b500 units for 6 months, three times a week, meanwhile with Zhuling polysaccharide of 4 mL daily for 2 months, to observe the variations of HBV DNA, HBeAg and HBeAg/anti HBe. Results In 32 samples, second-generation family genotypes were 37.5%, 50% and 12.5% respectively in B, C type and B, C mixed type, and the effect of combined treatment had no statistic difference in genes of sub-types. Conclusion Among different types of chronic hepatitis patients, variations exist between the percentage of genotypes B and genotypes C. It can be seen from the genotype changes in the second generation that genetic sub-typing is related to and natural variability exists among genetic sub-types.

关 键 词：乙型肝炎 基因型 基因亚型 

分 类 号：R512.62[医药卫生—内科学]