阿托伐他汀对氧化低密度脂蛋白诱导兔脂肪细胞表达凝血和纤溶因子的调节  

Atorvastatin modulates expression of thrombotic and fibrinolytic factors induced by oxidized low-density lipoprotein in cultured adipocytes

作　　者：李洁琪[1] 赵水平[2] 方颖[1] 吴立荣[1] 李屏[1] 

机构地区：[1]贵阳医学院附属医院心内科,贵州贵阳550004 [2]中南大学湘雅二医院心内科,湖南长沙410011

出　　处：《中华老年心脑血管病杂志》2006年第12期843-846,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：贵州省科技基金资助(2005-2066);贵州省省长基金资助(2005-224)

摘　　要：目的探讨阿托伐他汀对氧化低密度脂蛋白(OX-LDL)诱导兔脂肪细胞分泌组织因子(TF)和纤溶酶原激活物抑制剂1(PAI-1)的影响。方法取正常兔脂肪组织分离培养脂肪细胞,实验设对照组、ox-LDL干预组、阿托伐他汀干预组及联合干预组,除对照组外,其余3组分别以不同终浓度的ox-LDL和阿托伐他汀进行单独或联合干预,孵育24h。用酶联免疫吸附法检测TF和PAI-1蛋白含量。逆转录聚合酶链反应测定脂肪细胞TF和PAI-1 mRNA表达。结果ox-LDL使脂肪细胞分泌TF和PAI-1显著增加,TF和PAI-1 mRNA表达升高,并呈剂量依赖性。阿托伐他汀呈浓度依赖性地抑制兔脂肪细胞TF下和PAI-1 mRNA表达及蛋白产生。联合干预组与ox-LDL组比较,阿托伐他汀使TF和PAI-1 mRNA表达及蛋白分泌降低,但仍高于对照组。结论阿托伐他汀对ox-LDL诱导的兔脂肪细胞表达凝血和纤溶因子异常具有显著的调节作用。To study the effects of atorvastatin on expression of tissue factor （TF） and plasminogen activator inhibitor-1 （PAI-1） induced by oxidized low-density lipoprotein （ox-LDL） in cultured adipocytes. Methods Cultured adipocytes from normal rabbits （ n = 4） were incubated for 24 h in the presence of ox-LDL and atorvastatin at different concentrations either separately or in combination. TF and PAI-1 protein levels were determined with enzyme linked-immunosorbent assay. TF and PAI-1 mRNA were measured by reverse transcription-polymerase chain and PAI-1 release and up-regulated reaction. Results In adipocytes, ox-LDL dose-dependently increased TF their mRNA levels. Atorvastatin dose-dependently decreased TF and PAI-1 release and their mRNA levels. In the presence of ox-LDL, atorvastatin lowered the increased levels of TF and PAI-1 protein and their mRNA in adipocytes. Conclusion Atorvastatin effectively modulates the expression of thrombotic and fibrinolytic factors induced by ox-LDL in adipocytes.

关 键 词：脂细胞 脂蛋白类 LDL 纤溶酶原激活物抑制物1 阿托伐他汀 

分 类 号：R96[医药卫生—药理学]