一氧化氮合酶在慢性饮酒大鼠胃黏膜适应性细胞保护中的作用及其与乙醇体积分数的关系  

Effect of nitricoxide synthase on chronic alcohol drinking-induced adaptive cytoprotection of rat gastric mucosa and its relationship with volume fraction of ethanol

作　　者：陈敏[1] 杜军[2] 戈应滨[2] 林琳[1] 

机构地区：[1]南京医科大学附属第一医院消化内科 [2]南京医科大学生理学系,江苏省南京市210029

出　　处：《中国临床康复》2006年第48期133-135,F0003,共4页Chinese Journal of Clinical Rehabilitation

基　　金：江苏省"135工程"重点人才基金项目(RC2003087);江苏省自然科学基金资助项目(BK2004158).~~

摘　　要：目的:观察不同体积分数的乙醇刺激对慢性饮酒大鼠胃黏膜一氧化氮合酶活性的影响及其在适应性细胞保护作用中的意义。方法:实验于2004-09/2005-12在南京医科大学生理学系实验室进行。取健康雄性SD大鼠60只单纯随机分为6组,每组10只:正常对照组用自来水,其余5组分别以体积分数为0.01,0.03,0.06,0.12,0.24的乙醇水溶液作为大鼠饮水的惟一来源。在饮酒3d后,每组随机取7只动物,胃内灌注2mL的体积分数为1的乙醇,2h后和另外3只同时处死,观察大鼠胃黏膜的损伤情况(用损伤指数表示,评分越高,损伤越重),同时用免疫组化和反转录-聚合酶链反应方法观察各组大鼠胃黏膜一氧化氮合酶(包括神经型、内皮型和诱导型)及其mRNA的表达。结果:60只大鼠全部进入结果分析。①胃黏膜损伤指数:饮用体积分数为0.06,0.12乙醇组低于正常对照组(22±9,24±9,112±26,P<0.05),前2组间比较差异无显著性意义;饮用体积分数为0.01,0.03和0.24乙醇组与正常对照组比较差异无显著性意义(P>0.05)。②免疫组化染色结果:3种一氧化氮合酶的免疫组化阳性细胞在各组均有表达。③一氧化氮合酶mRNA的表达:内皮型一氧化氮合酶mRNA在各组大鼠的胃黏膜内的表达无规律;神经型一氧化氮合酶mRNA在饮用体积分数为0.06,0.12乙醇组几乎无表达,而在其他几组均有表达,且表达量没有明显差别;而诱导型一氧化氮合酶mRNA仅在饮体积分数为0.12,0.24乙醇组有表达。结论:慢性饮酒大鼠胃黏膜的神经型一氧化氮合酶的表达下降可引起胃黏膜的适应性细胞保护作用并和饮用乙醇的体积分数有关。AIM： To investigate the effect of ethanol in different volume fractions （VFs） on the activity of nitricoxide synthase （NOS） in the rats with chronic alcohol drinking and its role in adaptive cytoprotection in rat gastric mucosa. METHODS： The experiment was carried out in the Department of Physiology, Nanjing Medical University between September 2004 and December 2005. Sixty healthy male SD rats were randomly divided into six groups with ten rats in each group： In control group, rats drunk tap water, while in other five groups, rats received the drinking containing 0.01, 0.03, 0.06, 0.12 or 0.24 VF of ethanol respectively. After three days of drinking, seven rats were selected from each group to be perfused with 2 mL pure ethanol of 1 VF into the stomachs. All rats were decapitated at 2 hours after perfusion, containing the other three rats, and the severity of mucosal lesions were measured （The higher scores indicated the severer injury）. The expressions of three NOSs, including neuronal NOS （nNOS）, endothelial NOS （eNOS） and inducible NOS （iNOS） as Well as their mRNA in gastric mucosa were measured by semi-quantitative reverse transcriptase polymerase chain reaction （RT-PCR） and immunohistochemistry. RESULTS： Totally 60 rats entered the result analysis. ①Index of gastric mucosal injury： Compared with control group, the gastric lesions in rats were reduced after their exposure to the ethanol in 0.06 and 0.12 VF （22 ±9, 24±9, 112±26, P 〈 0.05）. And there was no significant difference between control rats and rats which were exposed to the ethanol in VF of 0.01, 0.03 and 0.24 （P 〉 0.05）.②Immunohistochemical staining： Three kinds of NOS were positively expressed in control and drinking groups.③ NOS mRNA expression： eNOS mRNA was expressed irregularly in all groups, nNOS mRNA was expressed equally in the control, 0.01, 0.03 and 0.24 VF of ethanol groups, while not expressed in 0.06 and 0.12 VF ethanol groups, iNOS mRNA was only expressed in ethanol groups

关 键 词：胃粘膜/损伤 一氧化氮合酶 乙醇 细胞保护 

分 类 号：R333.2[医药卫生—人体生理学]