精索静脉曲张大鼠附睾线粒体钙线粒体与细胞质细胞色素C含量变化及细胞的凋亡(英文)  

Changes of mitochondria calcium and cytochrome C in epididym is associated with apoptosis in varicocele rats

作　　者：马小茹[1] 王淑秋[1] 周成福[2] 刘月霞[1] 秦文波[2] 王淑湘[1] 

机构地区：[1]佳木斯大学基础医学院,黑龙江省佳木斯市154007 [2]佳木斯大学附属第一医院,黑龙江省佳木斯市154007

出　　处：《中国临床康复》2006年第48期200-203,207,共5页Chinese Journal of Clinical Rehabilitation

基　　金：黑龙江省自然科学基金(D00-55)~~

摘　　要：背景:精索静脉曲张可导致男性不育,探讨其机制对治疗男性不育有着重要意义。目的:分析外科所致的精索静脉曲张对大鼠附睾细胞线粒体钙、细胞色素C含量及附睾细胞凋亡和其显微、超微结构的影响。设计:随机对照实验。材料:实验于2003-06/2004-05在佳木斯大学完成。选取青春期雄性Wistar大鼠40只,体质量(220±20)g,室温下饲养1周后,随机分为假手术组、结扎组,每组20只。方法:假手术组显露左肾静脉建立假手术模型,结扎组部分结扎左肾静脉建立精索静脉曲张模型。术后10周,取双侧附睾,检测附睾头、体部细胞线粒体钙、细胞色素C含量及细胞质细胞色素C含量。以末端脱氧核糖核酸转移酶介导的dUTP缺口末端标记法(TUNEL)检测细胞凋亡,常规制作附睾体部光镜、电镜标本,观察附睾组织细胞形态变化。主要观察指标:细胞线粒体钙、细胞色素C含量;细胞质细胞色素C含量;细胞凋亡;附睾组织细胞形态变化。结果:40只大鼠全部进入结果分析。①结扎组左右侧附睾细胞线粒体钙含量较假手术组显著降低[(4.72±1.45),(5.90±1.97),(10.13±2.34)mg/g,P<0.01],②结扎组左右侧附睾细胞线粒体细胞色素C含量较假手术组线粒体组显著增高[(0.36±0.20),(0.19±0.14),(0.15±0.07)μmol/LP<0.05]。③结扎组左右侧附睾细胞胞质内细胞色素C含量较假手术组显著增高[(8.17±1.49),(7.48±1.60),(5.93±1.60)mol/L,(P<0.05)④结扎组左右侧细胞凋亡率较假手术组显著增高[(13.3±1.9)%,(12.6±1.5)%,(6.2±0.3)%,P<0.01]。结扎组左右侧线粒体钙含量、细胞色素C、胞质细胞色素C、凋亡率差别均无显著性意义(P>0.05)。⑤光镜下主要改变有附睾管萎缩,上皮细胞出现空泡化,(下转第207页)上皮内晕、亮细胞数明显增多。⑥电镜下主要表现为主细胞内溶酶体增多、变大,残余小体增加,内质网扩张,线粒体嵴模糊,高尔基复合体空泡化;核染色质BACKGROUND： Varicocele （VC） can induce the infertility in males, so the investigation on its mechanism is important for the treatment of male infertility. OBJECTIVE： To analyze the effects of VC induced by surgical operation on the contents of mitocbondria calcium, cytochrome C and cell apoptosis as well as the changes of microstructure and uhrastructure in epididymis. DESIGN： Randomized control experiment. MATERIALS： The experiment was conducted in Jiamusi University between June 2003 and May 2004. Forty male adolescent Wistar rats with the average body mass of （2203=20） g were selected, which were provided by the Animal Laboratory of Jiamusi University. Rats were randomly divided into sham-operation group and deligation group with 20 rats in each group at one week after feeding at room temperature. METHODS： Rats in the sham-operation group were made into sham-operation models by exposing the left renal vein. Rats in the deligation group were deligated of partial left renal veins so as to establish VC models. Bilateral epididymides were removed at ten weeks after operation. The levels of mitocbondria calcium in head and body of epididymis as well as the contents of cytochrome C and cytoplasm cytochrome C were detected. The cell apoptosis was detected by in situ terminal deoxynucleotityl transferasemediated dTUP nick end labeling （TUNEL） technique. The specimens of corpus epididymis were routinely made for observation under optimal microscopo and electron microscope. The changes of microstructure and ultrastructure of epididymis were studied. MAIN OUTCOME MEASURES： The contents of mitochondria calcium, cytochrome C and cytoplasm cytochrome C. Cell apoptosis. Changes of cellular morpbous in epididymis. RESULTS： A total of 40 rats were involved in the analysis of results.① The contents of mitochondria calcium in bilateral epididymis were obviously decreased in the deligation group than the sham-operation group [（4.72±1.45）, （5.90±1.97）, （10.13±2.34） rag/g, （P 〈 0.01 �

关 键 词：精索静脉曲张 附睾 线粒体 细胞色素C 细胞凋亡 

分 类 号：R697.24[医药卫生—泌尿科学]