荧光标记引物miniCTTA复合扩增系统的建立  

Establishment of miniCTTA multiplex set with fluorescence-labeled primers

作　　者：刘超[1] 王会品[2] 孙宏钰[3] 王慧君[1] 

机构地区：[1]南方医科大学法医学研究所,广东广州510515 [2]广州市刑事科学技术研究所,广东广州510030 [3]中山大学法医学系,广东广州510080

出　　处：《南方医科大学学报》2006年第11期1574-1576,共3页Journal of Southern Medical University

基　　金：国家自然科学基金(30400519);广州市重大科技攻关项目(2005Z1-E0051)~~

摘　　要：目的建立扩增片段<130bp,包括CSF1PO、TH01和TPOX及性染色体amelogenin基因座的miniCTTA扩增系统。方法采用不同荧光染料标记引物,通过PCR扩增,利用ABI3100遗传分析仪进行片段长度分析,对100份无关个体血样,10个家系样本以及30份高度降解检材进行检测。结果miniCTTA系统DNA分型结果与AmpFLSTRIden-tifiler试剂盒完全一致。结论miniCTTA系统可以应用于个人识别和亲权鉴定,为降解DNA样本分型提供了新的方法。Objective To establish a miniCTTA multiplex set including short tandem repeat （STR） markers as CSF1PO, TH01, and TPOX and amelogenin gene, whose amplified fragments are shorter than 130 bp. Methods The length of the gene fragments amplified with fluorescence-labeled primers were analyzed by ABI3100 Genetic Analyzer for genotyping of 100 unrelated individuals, 10 genealogies and 30 highly degraded specimens. Results The genotypes derived form miniCTTA were consistent with the results by AmpFLSTR Identifiler kit. Conclusion MiniCTTA multiplex set is useful for personal identification and paternity test, especially applicable for degraded DNA sample.

关 键 词：MINISTR 复合扩增 CSF1PO TH01 TPOX 性染色体 

分 类 号：D919[医药卫生—法医学]