IL-1β激活后的许旺细胞与人发角蛋白三维培养构建人工神经桥接体  

Construction of artificial nerve bridge by three-dimensional culture of interleukin-1β-activated Schwann cells with human hair keratins

作　　者：杨俊[1] 邱小忠[2] 余磊[2] 朴英杰[2] 秦建强[2] 

机构地区：[1]解放军188医院广州军区创伤骨科中心,广东潮州521000 [2]南方医科大学临床解剖学研究所,广东广州510515

出　　处：《南方医科大学学报》2006年第11期1577-1582,共6页Journal of Southern Medical University

基　　金：广东省科技计划重大专项(2003A3020101);广东省重点实验室(2001B60107)~~

摘　　要：目的探索白细胞介素-1β(IL-1β)激活后的许旺细胞与人发角蛋白复合培养构建人工神经桥接体,修复神经缺损。方法体外培养纯化的许旺细胞经IL-1#激活前、后与细胞外基质凝胶修饰的人发角蛋白进行三维方向的共培养而构建成人工神经桥接体,移植入SD大鼠坐骨神经缺损处。S-100蛋白免疫组化染色鉴定许旺细胞,倒置显微镜和扫描电镜下观察许旺细胞与人发角蛋白共培养情况,原位杂交法观察人发角蛋白降解及神经生长因子(NGF)在坐骨神经内的表达,移植术4周后检测坐骨神经功能指数。结果经IL-1$激活后的许旺细胞能更好地黏附于人发角蛋白表面,进行分裂、增殖。3～4周,桥接体内的人发角蛋白开始降解,其周围可见IL-1%激活后的处于增殖分裂期的许旺细胞NGF表达强阳性,坐骨神经功能恢复时间提前。结论经IL-1&激活后的许旺细胞与细胞外基质凝胶修饰的人发角蛋白复合培养构建成人工神经桥接体,具有修复神经缺损的同时还能够加速坐骨神经损伤后功能的恢复。Objective To culture interleukin-1 β （IL-1β）-activated Schwann cells （SCs） with human hair keratins （HHKs） for artificial nerve bridge construction. Methods SCs purified by primary culture with or without IL-1β activation were cultured with HHKs decorated by extracellular matrix （ECM）, and the artificial nerve bridge was implanted into the defect of rat sciatic nerve. The morphology of the SCs cultured with HHKs was monitored by inverted microscope, scanning electron microscope and evaluated by immunocytochemical staining, and the expression of nerve growth factor （NGF） in the sciatic nerve was observed by in situ hybridization. Results Activated SCs showed better ability to adhere to the HHKs and grew well. The HHKs component in the artificial nerve bridge underwent degradation in the sciatic nerve defect after 3 to 4 weeks, and IL1β activation resulted in enhanced NGF expression in the SCs. Conclusion The constructed artificial nerve bridge by three-dimensional culture of IL-1β-activiated SCs with HHKs decorated by ECM promotes the repair of sciatic nerve defects and accelerates sciatic nerve regeneration.

关 键 词：白介素-1Β 许旺细胞 人发角蛋白 周围神经 组织工程 

分 类 号：R318[医药卫生—生物医学工程]