慢性乙型肝炎患者CXC趋化因子Mig的表达  

Expression of chemokine monokine induced by interferon-γ in patients with chronic hepatitis B

作　　者：赵金红[1] 王健[1] 江水清[2] 项桂菊[2] 

机构地区：[1]安徽理工大学医学院病原学与免疫学教研室,安徽淮南232001 [2]淮南第二矿工医院感染科,安徽淮南232057

出　　处：《南方医科大学学报》2006年第11期1589-1592,共4页Journal of Southern Medical University

基　　金：安徽省教育厅自然科学基金重点项目(2003kj027zd)~~

摘　　要：目的探讨慢性乙型肝炎(乙肝)患者CXC趋化因子Mig的表达水平及其与HBV-DNA、丙氨酸转氨酶(ALT)的相关性。方法以实时PCR法动态检测慢性乙肝患者外周血单个核细胞(PBMC)内MigmRNA的表达水平,以趋化因子与GAPDH比值为其最终含量。以ELISA法定量检测患者外周血中Mig蛋白含量。结果慢性乙肝患者PBMC内MigmRNA表达水平为0.6883±0.0693,与正常对照组相比差异有显著性(P<0.001)。Mig血清浓度为(609.6±73.8)pg/ml,与正常对照组相比差异有显著性(P<0.05)。患者PBMC内MigmRNA与其外周血中Mig含量显著相关(r=0.7157,P<0.001),外周血中Mig含量与血清ALT水平显著相关(r=0.7220,P<0.001),外周血中Mig含量与血清中HBV-DNA含量显著相关(r=0.7266,P<0.001)。结论慢性乙肝患者MigmRNA及血清Mig含量表达均增高,Mig介导了肝脏炎症细胞浸润,参与了乙肝慢性化病程。Objective To study the expression levels ofmonokine induced by interferon-γ （Mig） mRNA and its association with HBV DNA and alanine aminotransferase （ALT） in patients with chronic hepatitis B. Methods The level ofMig mRNA in peripheral blood mononuclear cells （PBMCs） was dynamically detected with real-time quantitative PCR, and the ratio of chemokine/GAPDH was considered to represent the final chemokine level. The plasma level of was measured by enzyme-linked immunosorbent assay （ELISA）. Results The mean level of Mig mRNA in PBMCs of the patients with chronic hepatitis B was 0.6883±0.0693, which was significantly higher than that in normal controls （P〈0.001）. The plasma Mig level in the patients was 609.6±73.8 pg/ml, also significantly higher than that in normal controls （P〈0.05）. In patients with chronic hepatitis B, the level ofMig mRNA in the PBMCs was significantly correlated with plasma Mig level （r=0.7157, P〈0.001）, and plasma Mig level was correlated with plasma ALT level （r=0.7220, P〈0.001） and plasma HBV DNA level （r=0.7266, P〈0.001）. Conclusion Both the expression ofMig mRNA in PBMCs and plasma Mig concentration are elevated in patients with chronic hepatitis B. Mig plays an important role in migration of the inflammatory cells to the liver and mediates the development of chronic hepatitis B .

关 键 词：乙型肝炎 趋化因子 MIG 丙氨酸转氨酶:实时PCR 

分 类 号：R512.62[医药卫生—内科学]