益母草类中药原植物的核核糖体内转录间隔区序列分析  

Nuclear ribosomal DNA internal transcribed spacer 1 sequences of 4 Leonurus species

作　　者：杨志业[1] 晁志[2] 霍克克[3] 吴炳义[4] 潘胜利[1] 

机构地区：[1]复旦大学药学院,上海200032 [2]南方医科大学中医药学院,广东广州510515 [3]南方医科大学南方医院临床实验研究中心,广东广州510515 [4]复旦大学生命科学院,上海200032

出　　处：《南方医科大学学报》2006年第11期1593-1595,共3页Journal of Southern Medical University

基　　金：广州市科技计划项目(2002J1-C0161)~~

摘　　要：目的分析益母草属4种植物nrDNAITS序列,探讨其在益母草药材DNA分子鉴别中的作用。方法对4种益母草属植物分别进行ITS序列的PCR扩增和测序,并运用CLUSTRALX和MEGA软件进行分析。结果测得4种植物的ITS序列,包括ITS1、5.8S和ITS2全长序列以及18S、26S部分序列;益母草及其易混淆的3种植物ITS1、ITS2序列的差异性分别为7.2%～18.8%和14.2%～27%。根据ITS序列特征所构建的系统树与传统分类有所不同。结论ITS序列特征可作为益母草种间鉴别的有效分子标记。Objective To analyze the nuclear ribosome DNA （nrDNA） internal transcribed spacer （ITS） sequences of 4 Leonurus species, and the possibility of using them for molecular authentication of the crude drugs from the genus. Methods The nrDNA ITS sequence （including ITS1, 5.8S rDNA, ITS2, and partial 18S rDNA and 26S rDNA） ofL. japonicus and its 3 adulterant species were amplified and sequenced, and CLUSTRAL X and MEGA software was employed for analysis. Results The variation of ITS1 and ITS2 between L. japonicus and its adulterant species ranged between 7.2% and 18.8% and between 14.2% and 27%, respectively. The phylogenic tree derived from the dendrograms based on the ITS sequence data contained some discrepancy from the traditional classification. Conclusion The nrDNA ITS sequences can be used potentially as efficient markers for identification of L. japonicus and its adulterants, and further study is needed for studying the phylogeny of Leonurus.

关 键 词：益母草属 ITS 序列分析 核核糖体 

分 类 号：R284[医药卫生—中药学]