重组β-防御素2在脓毒症所致大鼠急性肺损伤发生发展中的作用  

Protective effect of recombinant β-defensin-2 on acute lung injury induced by sepsis in rats

作　　者：石卓[1] 赵正言[1] 舒强[1] 陈智[2] 姚航平[2] 方向明[3] 

机构地区：[1]浙江大学医学院附属儿童医院,浙江杭州310003 [2]浙江大学医学院附属第一医院传染病研究所,浙江杭州310003 [3]浙江大学医学院附属第一医院麻醉科,浙江杭州310003

出　　处：《浙江大学学报（医学版）》2006年第6期605-609,共5页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金项目(A30070854);浙江省科技厅基金项目(001103241)

摘　　要：目的:探讨重组β-防御素2(BD 2)对脓毒症所致大鼠急性肺损伤的保护作用以及对预后的改善。方法:SD大鼠36只随机分为对照组及实验组,每组各18只,分别予以气管滴注对照腺病毒载体(A d-V,107PFU/m l)及可表达大鼠β-防御素2(rBD 2)的腺病毒载体(A d-rBD 2,107PFU/m l)各100μl。48 h后行盲肠结扎穿孔复制脓毒症模型;复制此模型后36h处死大鼠(n=12,实验组、对照组各6只),行腹腔灌洗液细菌计数、肺组织常规病理切片观察以及大鼠生存率分析(n=24,A d-V组、A d-rBD 2组各12只)。结果:与对照组(A d-V组)比较,实验组(A d-rBD 2组)肺组织损伤程度减轻,生存率明显提高(P<0.05),腹腔灌洗液细菌计数无明显差别。结论:在体内表达的重组β-防御素2可明显改善脓毒症所致的急性肺损伤的肺部病变及其预后。Objective： To explore the protective effect of recombinant β-defensin-2 （BD2） on acute lung injury induced by sepsis in rats. Methods： Recombinant adenovirus carrying an expression cassette of rat BD2 （rBD2） or control adenovirus carrying empty vector was administered intratracheally to Sprague-Dawley rats 48 h before acute lung injury, which was induced by cecal ligation and double puncture （CLP）. Histological changes of the lungs and 7-day survival rate were determined. Results： The alveolar damage, interstitial edema, and infiltration of neutrophils were reduced and the survival rate was increased significantly in BD2 group than those in control group （P〈0.05）. The CFU of abdominal bacteria was comparable to that in the control rats （P〉0.05）. Conclusion： The administration of recombinant BD2 protects lung injury and improves the survival of rats with induced sepsis.

关 键 词：防御素类/治疗应用 脓毒症/治疗 脓毒症/病理学 基因疗法 疾病模型 动物 Β-防御素 抗菌肽 腺病毒表达载体 急性肺损伤 盲肠结扎穿孔 预后 

分 类 号：R459.7[医药卫生—急诊医学]