采用柱层析分离与富集虫草素研究  

Research on separation and enrichment of cordycepin by column chromatography

作　　者：陈伟[1] 吴畏[1] 高新华[1] 唐乐珊[1] 沈明[1] 张红霞[1] 

机构地区：[1]上海市农业科学院植物保护研究所,上海201106

出　　处：《上海农业学报》2006年第4期69-71,共3页Acta Agriculturae Shanghai

基　　金：上海市农科院科学发展基金[农科发2004(8)];上海市科学技术发展基金(01JG0535);国家农业科技成果转化资金项目(03EFN213100091);国家级星火计划项目(2004EA680017)

摘　　要：以反相高效液相色谱全程监控虫草提取物中虫草素含量变化情况。研究结果表明,乙醇沉淀物中虫草素占总量的2%,乙醇上清液中虫草素含量占总量的98%,确定乙醇上清液可以作为样品;采用硅胶柱层析可以高效率地分离和富集虫草素。经硅胶柱层析,虫草素相对含量从1.40‰提高到20.89‰,为富集前的15倍;虫草素的硅胶色谱柱的最佳溶剂系统为,氯仿∶乙酸乙酯∶异丙醇∶水=400∶100∶300∶24,每10 mL流动相中加入2滴氨水;此法可以作为大量制备高纯度虫草素的产业化技术。The cordycepin content of extracts from Cordyceps militaris was monitored by HPLC. The result showed that the cordycepin was 296 in the precipitate and 98 % in the ethanol, and the ethanol was used as a sample. Using column chromatography could efficiently separate and enrich the cordycepin. The content of cordycepin went up from 1.40‰ to 20.89‰, 15 times as much as before enrichment. The optimum solvent of column chromatography was chloroform： ethyl acetate： isopropyl alcohol ： water = 400 ： 100 ： 300：24, and two drops of ammonium hydroxide were added to every 10 mL mobile phase, which could be used as an industrial technology of preparing a large amount of high-purity cordycepin.

关 键 词：虫草素 柱层析 分离 富集 

分 类 号：S567.35[农业科学—中草药栽培]