高赖氨酸蛋白基因遗传转化水稻的研究  

Research of transforming Lysine-rich protein gene to rice

作　　者：蒋家焕[1] 刘峰[1] 许明[1] 黄志伟[1] 谢丽雪[1] 郑金贵 

机构地区：[1]福建农林大学农产品品质研究所

出　　处：《福建农林大学学报（自然科学版）》2006年第6期615-618,共4页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：科技部国家重大基础研究前期研究专项(2005CCA01300);国家自然科学基金项目(30471073;30671276);福建省科技厅重大专项前期研究项目(2005NZ1022);福建省科技厅重大项目(2001Z012)资助

摘　　要：采用农杆菌介导的方法将高赖氨酸蛋白基因导入台粳9号幼胚诱导的胚性愈伤组织中,经潮霉素筛选,抗性愈伤分化成苗,共获得2株转基因植株.对这些植株及后代植株进行GUS染色和PCR及PCR Southern b lot检测分析,确定高赖氨酸蛋白基因已整合到台粳9号基因组中,并能稳定遗传表达.经检测,转基因水稻糙米赖氨酸含量为0.349%,比对照提高29.3%;转基因水稻秸秆赖氨酸含量为0.341%,比对照提高8.3%.Lysine-rich protein gone was transformed to the embryonic callus tissue of Taijing 9 with Agrobacterium-mediated method. After resistance screening by hygromix, the resistant calli differentiated to seedlings, and 2 transgenic plants were obtained. PCR testing and GUS staining analysis showed that Lysine-rich protein gene had been transformed to the genome of Taijing 9, and it could be stably expressed in its next generation. The test results showed that lysine content in unpolished grains of transgenie rice was 0.349%, improved 29.3% compared to control; and lysine content in transgenic rice straw was 0.341%, enhanced 8.3% compared to control.

关 键 词：台粳9号 高赖氨酸蛋白基因 遗传转化 农杆菌 

分 类 号：Q785[生物学—分子生物学]