人类免疫缺陷病毒1 Gag-特异性T淋巴细胞增殖反应特征的研究  

Characterization of HIV-1 Gag-specific proliferative T cell responses in Chinese HIV-1 infected patients

作　　者：王琪[1] 尚红[1] 王亚男[1] 张子宁[1] 姜拥军[1] 

机构地区：[1]中国医科大学第一临床学院卫生部艾滋病免疫学重点实验室,沈阳110001

出　　处：《中华医学杂志》2006年第44期3099-3103,共5页National Medical Journal of China

基　　金：国家"十五"科技攻关课题资助项目(2004BA719A12);国家自然科学基金资助项目(30471548);科技部重大基础研究前期研究专项基金资助项目(2004CCA02000)

摘　　要：目的了解人类免疫缺陷病毒(HIV)感染者HIV-1Gag-特异性T淋巴细胞增殖反应特征,分析它们与疾病进展的关系,探讨HIV特异性免疫功能缺陷的发生机制。方法采用BrdU掺入的流式细胞仪胞内染色法,检测34例HIV-1感染者PBMC对HIV-1Gag抗原的特异性增殖反应频率,分析它们与CD4+T细胞绝对值、血浆病毒载量及CD8+T细胞活化指标的相关性。结果HIV感染者HIV-1Gag-特异性CD4+T细胞增殖率显著低于健康对照组(P<0·01),与CD8+T细胞CD38表达负相关(P<0·01),与CD4绝对值正相关(P<0·01),与血浆病毒载量负相关(P<0·05);中国HIV感染者HIV-1Gag-特异性CD8+T细胞增殖率显著高于健康对照组(P<0·01),与CD8+T细胞CD38表达正相关(P<0·01),与CD4绝对值显著负相关(P<0·01),与血浆病毒载量不相关(P>0·05)。结论HIV感染者T细胞增殖功能异常,HIV-1Gag-特异性CD4+T细胞增殖功能减低,其程度与疾病进展密切相关。Objective To investigate the characteristics of HIV-1 Gag-specific T lymphocyte proliferative responses in Chinese HIV infected patients, analyze their associations with disease progression and explore the mechanism of the HIV-specific immune dysfunction in HIV infection. Methods Specific proliferative responses of PBMC from thirty four HIV-1 infected patients to HIV-1 Gag antigen were detected by a BrdU incorporated flow cytometry intracellular stain, their relations with CD4^ ＋ T cells absolute counts, plasma viral loads and CD8^ ＋ T cell activate status were analyzed. Results HIV-1 Gag-specific CD4^ ＋ T cell proliferative responses of HIV-infected patients were significantly lower than that of healthy controls （ P 〈 0. 01 ）, correlated inversely to CD38 expression on CD8^ ＋ T lymphocytes and plasma viral loads （ P 〈 0. 05, P〈0. 05）, and directly to CD4^＋T cell absolute counts （P 〈 0. 01 ）. HIV-1 Gag-specific CD8^＋T cell proliferative responses of HIV-infected patients were significantly higher than that of healthy controls （ P 〈 0. 01 ）, correlated directly to CD38 expression on CD8 ^＋ T lymphocytes （ P 〈 0. 05 ） , and inversely to Cd4^＋ T cell absolute counts（P〈0. 01）, but not correlated to viral loads （P 〉0. 05）. Conclusion HIV-1 Gagspecific T cell proliferative response were abnormal in Chinese HIV-1 infected persons, HIV-1 Gag-specific CD4^ ＋ T cell proliferative responses were decreased and their frequencies were closely correlated with disease progression.

关 键 词：HIV-1 CD4阳性T淋巴细胞 抗原 CD8 

分 类 号：R392[医药卫生—免疫学]