猪乙脑快速诊断方法的建立与应用  

Development and Application of Rapid Diagnosis for Japanese Encephalitis Virus in Swine

作　　者：唐青海[1] 乔宪凤[2] 安立国[1] 周荆荣[2] 郑新民[2] 刘西梅[2] 华文君[2] 何维明[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]湖北省农业科学院畜牧兽医研究所,武汉430064

出　　处：《湖北农业科学》2006年第6期703-705,共3页Hubei Agricultural Sciences

基　　金：湖北省自然科学基金项目(2005ABA195)

摘　　要：根据乙脑病毒基因组3′末端保守区设计一对特异性引物,建立了乙脑病毒不同株的RT-PCR诊断技术。该技术可以从乙脑病毒WHe株、SA14-14-2株、P3株、SA14-14-2VS株接种的鼠脑组织中扩增出486bp的核酸片段,检出的灵敏度约为2pg。对20份临床样品的检测结果与小鼠脑内接种试验(MIT)的测定结果完全吻合,但RT-PCR诊断技术可在3h内直接对组织进行诊断,具有敏感、快速、稳定的优点。Specific primers were designed according to the published nucleotide sequences of SA14 （U14163） and partial determined nucleotide sequences of JEV WHe strain, a reverse transcription and polymerase chain reaction （RT-PCR） technique for viral nucleic acids as an alternative protocol for diagnosis of Japanese encephalitis virus were developed.20 brain samples from the swines which suffered from abortion, stillborn delivery or mummified fetuses, were detected by the RT-PCR and mouse inoculation test（MIT）. Compared with MIT and haemagglutination inhlbition（HI）, RT-PCR was more rapid and efficient than the MIT and HI to detect Japanese encephalitis virus.

关 键 词：乙脑病毒 反转录聚合酶链式反应 检测 

分 类 号：S852.659.6[农业科学—基础兽医学] Q503[农业科学—兽医学]