同种大鼠胚胎胰组织移植后在异体内的增殖和分化  

Organogenesis of allografted pancreatic anlagen

作　　者：王璐[1] 黄亚冰[1] 郭晖[1] 王树森[1] 谢林[1] 曾梦华[1] 李荣[1] 陈实[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院器官移植研究所教育部/卫生部重点实验室,武汉430030

出　　处：《中华器官移植杂志》2006年第12期740-743,共4页Chinese Journal of Organ Transplantation

基　　金：国家高新技术研究发展计划(863计划)基金资助项目(2003AA205009)

摘　　要：目的研究同种大鼠胚胎胰组织移植后在异体内重新血管化并再生长发育的可能性。方法将妊娠14．5 d(E14．5)和妊娠15．5 d(E15．5)的Lewis大鼠胚胎胰移植到成年健康Lewis大鼠的肾包膜下,分别在移植后3周和6周开腹观察移植胰的发育情况,并取标本作病理切片观察,采用免疫组织化学染色半定量和定位。结果(1)E14．5和E15．5的胚胎胰移植入肾包膜下3周后,体积均增大约10-15倍,外分泌部分腺泡导管分化发育,B细胞大量增殖,形成胰岛,组织周围有新生血管,且胰岛素分泌功能正常。(2)胚胎胰植入肾包膜下6周后,E14．5的胚胎胰内分泌部分进一步增殖;E15．5的胚胎胰则未见增殖,外分泌部分均有纤维化表现,内分泌部分与外分泌部分有分离趋势。结论(1)E14．5与E15．5的胚胎胰移植于同种大鼠肾包膜下均可在异体内再血管化,并发育为近似正常的胰腺组织。(2)E14．5和E15．5胎龄的大鼠胚胎胰都是比较适合移植的。Objective To study the possibility of revascularization, growth and differentiation of embryonic pancreatic anlagen transplanted to adult hosts. Methods The pancreas from embryonic day 14.5 and 15.5 Lewis rat embryos were implanted into the intra-peritoneal and sub-renal capsular site of healthy Lewis rats. At 3rd and 6th week after implantation, the pancreatic anlagen in host rats were resected for measurement, histopathological and immunohistochemical examinations. Results （1） At 3rd week after implantation into sub renal-capsular site, E14.5 and E15.5 pancreatic anlagen had enlarged 10-15 times. The acinar component had differentiated. The proliferation of the β cells was obvious, and several islets that stained positive for insulin could be found. There were some new vessels around the tissue. （2） At 6 th week after implantation into sub-renal capsular site, continued proliferation of the endocrine islets in E14.5 pancreatic anlagen could be observed, while the further proliferation couldn＇t seen on the E15.5 pancreatic anlagen. Fibrosis appeared in the exocrine component. It seemed that endocrine islets would separate from the exocrine component. （3） At 3rd week after implantation into intra-peritoneal site, E15.5 pancreatic anlagen had enlarged. β cells proliferated and formed the islets. However, neither the size of the pancreatic anlagen nor the proliferation of the β cells was more obvious than that in the sub renal capsular site. Conclusions （1） The allografted E14.5 and E15.5 pancreatic anlagen could revascularise and grow to tissues that seem like the ordinary pancreas. （2） E14.5 and E15.5 are adequate embryonic age for the transplantation of pancreatic anlagen. （3） Contrasted to the pancreatic anlagen transplanted into the intra-peritoneal site, anlagen trans- planted into the sub-renal capsular site is bigger in size and more β cells are observed.

关 键 词：胚胎组织移植 移植 同种 再血管化 

分 类 号：R657.5[医药卫生—外科学]