RP-HPLC测定开胸顺气滴丸中厚朴酚与和厚朴酚的含量  

Determination of magnolol and isomagnololin in Kaixiong Shunqi drops by RP-HPLC

作　　者：蒋平[1] 王文清[1] 王晨[1] 范耀[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,湖北武汉430030

出　　处：《华西药学杂志》2006年第6期577-579,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的 建立开胸顺气滴丸中厚朴酚与和厚朴酚含最的测定方法。方法 采用RP—HPLC法，色谱柱为VPC18（150mm×4．6mm，5μm）；流动相为甲醇-乙腈-水（50：20：40）；流速1.0ml·min^-1；柱温25℃；检测波长294nm。结果 厚朴酚的线件范围为0．2961～2．9610μg（r=0.9996）；和厚朴酚的线性范围为0．1608—1．6080μg（r=0．9996）。厚朴酚的平均回收率为99.17％±1．66％，RSD=1．3％（n=6）；和厚朴酚的平均回收率为99．04％±2.06％，RSD=1．7％（n=6）。结论 所用方法简便、准确，可用于开胸顺气滴丸中厚朴酚与和厚朴酚的含量测定。OBJECTIVE To establish a method for determination of magnolol and isomagnololin in Kaixiong Shunqi drops. METH- ODS An RP - HPLC method was adopted. The analysis was carried out on an analytical column C18 （ 150 mm × 4. 6 mm,5 μm）. The mobile phase consisted of methanol -acetonitrile -water （50 ： 20 ： 40 ） and the flow rate was 1.0 ml·min^-1, The detective wavelength was set at 294 nm with column temperature of 25℃. RESULTS The lienar range was 0. 2961 - 2. 9610 μg （ r = 0. 9996） and the average recovery was 99.17%±1.66% with the RSD of 1.3% （n = 6） for magnolol, respectively. The linear range was 0, 1608 - 1. 6080 μg（ r = 0. 9996 ） and the average recovery was 99.04%± 2.06% with the RSD of 1.7% （ n = 6 ） for isomagnololin, respectively. CONCLUSION The method is simple,reproducible and suitable for determination of magnolol and isomagnololin in Kaixinng Shunqi drops.

关 键 词：开胸顺气滴丸 厚朴酚 和厚朴酚 RP—HPLC 含量测定 

分 类 号：R927.2[医药卫生—药学]