聚乙二醇修饰的蓖麻毒蛋白A链的免疫原性和在小鼠体内急性毒性研究  

Immunogenicity and Acute Toxicity of Ricin A-chain Modified by Polyethylene Glycol

作　　者：赵燕彪 侯黎伟 丁敏敏 张慧娥[2] 

机构地区：[1]山东弘立医学动物实验研究有限公司,山东济南250101 [2]山东省郓城县疾病预防控制中心,山东郓城274700

出　　处：《食品与药品》2006年第12A期41-43,共3页Food and Drug

摘　　要：目的研究单甲氧聚乙二醇(mPEG)与蓖麻毒蛋白A链(RA)偶联后RA免疫原性和毒性的改变。方法从天然蓖麻籽中提取纯化的RA,经与含活性马来酰亚胺基团的mPEG反应偶联,得到纯化的mPEG偶联RA。用蛋白质印迹方法比较RA修饰前后的免疫原性,通过测定半数致死量(LD50)比较RA修饰前后的毒性。结果mPEG偶联RA后免疫原性和毒性均明显下降。偶联前RA链的LD50为2.37 mg.kg-1,经mPEG修饰后RA的LD50为6.15mg.kg-1。结论聚乙二醇能遮蔽RA,降低其免疫原性和毒性。Objective To study the difference between ricin A-chain（RA） and ricin A-chain modified by polyethylene glycol（RA-mPEG） in immunogenicity and acute toxicity. Methods Monomethoxy polyethylene glycol （mPEG） was conjugated with RA by disulfide bond. The immunogenicity of RA and RA-mPEG were compared by Western blot method. And their toxicities were compared by LD50 values. Results The immunogenicity and toxicity of RA-mPEG were remarkably lower than RA. LD50） of RA was 2.37 mg · kg^-1, while LDso of RA-mPEG was 6.15 mg · kg^-1. Conclusion It＇ s probably because of the cover of PEG that the immunogenicity and acute toxicity of RA is decreased significantly.

关 键 词：蓖麻毒蛋白A链 免疫原性 急性毒性 

分 类 号：R967[医药卫生—药理学]