免疫-PCR法检测梅毒螺旋体特异性抗体  

Detection of Treponema pallidum Specific Antibody with Immuno-PCR

作　　者：史俊岩[1] 刘兵[1] 牟玲[2] 罗恩杰[1] 

机构地区：[1]中国医科大学基础医学院病原生物学教研室,辽宁沈阳110001 [2]沈阳市传染病院,辽宁沈阳110006

出　　处：《微生物学杂志》2006年第6期23-25,共3页Journal of Microbiology

基　　金：沈阳市科委资助项目(2001419-01)

摘　　要：以梅毒螺旋体重组蛋白为抗原,应用免疫-PCR方法检测梅毒螺旋体抗体,并同常规ELISA法进行比较,探讨免疫-PCR方法检测梅毒螺旋体特异性抗体的可行性。结果免疫-PCR法敏感性是常规ELISA法的104倍,阳性检出率高于ELISA法;对照血清标本梅毒螺旋体抗体检测为阴性。表明免疫-PCR方法具有较高敏感性和特异性,有一定的临床推广价值,对梅毒患者的早期诊断及时治疗等具有重要意义。In order to investigate the feasibility of immuno-PCR method to detect Treponema pallidum （Tp） specific antibody, its recombinant protein was used as antigen applying immuno-PCR method to detect its antibody, at the same time routine ELISA method was used as a comparison. The results showed that the sensitivity of immuno-PCR method was 10^4 times higher than that of routine ELISA method, and positive detected rate was higher than ELISA method ; and control serum sample of Tp antibody was detected negative. These indicated that immuno-PCR method possesses fairly high sensitivity and specificity and has a certain worth of extension in clinic, and is of great significance for early diagnosis and treatment of Tp patients.

关 键 词：免疫-PCR 梅毒螺旋体特异性抗体 ELISA 

分 类 号：R759[医药卫生—皮肤病学与性病学]