线粒体连续超薄切片电镜图像的三维可视化  

Visualization of 3D Structure of Mitochondria Reconstructed from Electron Microscopy of Serial Ultrathin Sections

作　　者：李晶 黄小俊[2] 阮兴云 李鲲鹏[2] 张景强[2] 

机构地区：[1]昆明总医院医学工程中心,云南昆明650032 [2]中山大学生命科学学院∥生物防治国家重点实验室,广东广州510275

出　　处：《中山大学学报（自然科学版）》2006年第6期72-74,共3页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省自然科学基金资助项目(05300638);中国博士后科学基金资助项目(2004035183)

摘　　要：研究建立对连续超薄切片电镜图像的三维可视化方法。对正常及用SARS冠状病毒感染非洲绿猴肾细胞(Vero E6)样品进行连续超薄切片,通过透射电镜得到线粒体断层图像,用表面显示技术对线粒体进行三维重构显示。正常和SARS冠状病毒感染的Vero E6细胞中的线粒体在结构上有明显区别,揭示出SARS冠状病毒感染Vero E6细胞线粒体的病理改变。以此建立起电镜连续超薄切片线粒体图像三维可视化方法。A novel visualization method of 3D （three- dimension＇d） mitochondria structures reconstructed from serial ultrathin sections transmission electron microscope （TEM） images which applies surface rendering technique was presented. In order to validate the method, mitochondria in control and the Veto E6 cells, which were infected with SARS Coronavirus, were prepared by serial uhrathin sections, imaged in electron microscopy then reconstructed from serial section images and the results were compared with each other. As a result, mitochondria in control and the Vero E6 cells infected with SARS have remarkable difference in 3D structures, that illuminates pathological changes of Veto E6 cells. The results also testify the visualization method is valid.

关 键 词：线粒体 表面显示 连续超薄切片 三维可视化 

分 类 号：Q6[生物学—生物物理学]