人工培养条件下松材线虫提取物的毒性研究  

STUDIES ON TOXICITY OF EXTRACTION OF PINE WOOD NEMATODES CULTIVATED IN ARTIFICIAL MEDIA

作　　者：曹越[1] 沈伯葵[1] 

机构地区：[1]南京林业大学森林资源与环境学院

出　　处：《南京林业大学学报（自然科学版）》1996年第4期13-16,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

摘　　要：将来源于黑松、马尾松的松材线虫分别用单异活体法和无异活体法人工培养后，以不同方法和不同溶剂对培养物进行提取，并用粗提浓缩液对３０ｄ以上生的黑松和马尾松幼苗做生物测定。结果表明。After multiplication in monoxenic and axenic culture media, pine wood nematodes isolated from naturally infected Pinus thunbergii and P. massoniana are extracted individually in different ways and solvents. Nematode extracts are assayed by application of extracts to over 30-day-old seedlings of P.thunbergii and P. massoniana. It is clarified that wilt toxins are not produced by pine wood nematodes in artificial culture conditions.

关 键 词：松材线虫 活体培养 毒素 生物测定 

分 类 号：S763[农业科学—森林保护学]