饥饿及再投喂对日本囊对虾蛋白质代谢的影响  

The effects of starvation and refeeding on protein metabolism in Marsupenaeus japonicus

作　　者：吴立新[1] 刘璐[1] 张晓雪[1] 刘瑜[1] 邓宏相[1] 

机构地区：[1]大连水产学院辽宁省省级高校水生生物学重点实验室,辽宁大连116023

出　　处：《大连水产学院学报》2006年第4期301-306,共6页Journal of Dalian Fisheries University

基　　金：辽宁省博士科研启动基金资助项目(20021049);教育部留学回国人员科研启动基金资助项目(教外司留[2005]383号);辽宁省教育厅高等学校科学研究项目资助(202132030)

摘　　要：研究了体重为（13．08±2．40）g、体长为（10．37±0．73）cm的日本囊对虾Marsupenaeus japonicus在饥饿和再投喂状态下蛋白质代谢的变化。对照组C连续饱食投喂10d，试验组S10、S15、S25分别饥饿10、15、25d后再恢复饱食投喂10d。结果表明：在饥饿状态下，日本囊对虾肌肉中蛋白质的含量在饥饿前15d迅速上升，饥饿第15～25d趋于平稳，肝胰脏中蛋白质的含量则一直较稳定；恢复投喂后，肌肉中蛋白质的含量下降，而肝胰脏中蛋白质的含量上升。血浆中蛋白质的含量在饥饿前期（0—5d）保持平稳，但在之后的第5—15d则迅速下降，最后又恢复至最初水平；恢复投喂后，除S25组外其余各组血浆中蛋白质的含量都呈上升趋势，且试验组显著高于对照组（P〈0．05）。肌肉中RNA／DNA的值在饥饿初期（0—5d）略有下降，之后保持稳定，肝胰脏中RNA／DNA的值则在短期下降后有所上升；恢复投喂后，肌肉中RNA／DNA的值除S25组外其余各组均呈上升趋势，而肝胰脏中RNA／DNA的值除C组外其余各组均显著上升（P〈0．05）。The changes in protein metabolism were studied in shrimp Marsupenaeus japonicus weight of （ 13.08 ± 2.40 ） g and total length of （ 10.37 ± 0. 73 ） cm during starvation and with initial wet refeeding. The shrimp in Group C was fed to satiety for 10 days, and the shrimp in Groups S10, S15 and S25 were deprived of food for 10, 15 and 25 days, respectively, and then fed to satiety for 10 days. The results showed that the starved shrimp had a rapid increase in protein content in muscles during the first 15 days, and then maintained this protein level in the following 10 days, but the protein content in the hepatopancreas was always constant. During the course of refeeding, the protein content decreased in the muscles, but increased in the hepatopan- creas. During the starvation period, the plasma protein levels kept stable at the first five days, and decreased during the subsequent 10 days （5 - 15 d） , and then recovered again. After refeeding, there were increase in plasma protein levels in all the groups except the group S25. There was significanty higher plasma protein lev- els in the treatment groups than in group C. Food deprivation caused a slightly decline in RNA/DNA ratio in the muscles at the first five days, and then kept constant, while there was an increase in RNA/DNA ratio in the hepatopanereas after short - time decline. After refeeding, the ratio of RNA/DNA in muscles increased gradually in all groups except Group S25, while the ratio of RNA/DNA in hepatopancreas was a significant increase in all groups except Group C.

关 键 词：日本囊对虾 饥饿 再投喂 蛋白质代谢 RNA/DNA 

分 类 号：S917[农业科学—水产科学]