ALA-PDT结合TGF-β1基因转染对结肠癌细胞抑制作用的研究  

The study the effect of ALA-PDT combined with human TGF-β1 gene transfection on proliferation of human colon carcinoma

作　　者：陈祖林[1] 刘嘉[1] 廖海洋[2] 潘英俊[2] 葛海燕 肖卫东[1] 

机构地区：[1]第三军医大学新桥医院普外科,重庆400037 [2]重庆大学光电技术及系统教育部重点实验室,重庆400044

出　　处：《激光杂志》2006年第6期89-90,共2页Laser Journal

基　　金：国家自然科学基金资助项目。批准号:30271481

摘　　要：目的:探讨ALA-PDT结合hTGF-β1基因转染对结肠癌细胞体外增殖的抑制作用。方法:以腺病毒为载体将hTGF-β1基因导入人结肠癌细胞株8W480后,行PDT处理,应用免疫组化法检测TGF-β1的表达情况。通过MTT法检测转染后细胞增殖的抑制情况,应用流式细胞技术检测细胞周期的变化。结果:转染hTGF-β1基因后,TGF-β1蛋白表达明显增强。与单纯行PDT相比,G0/S周期阻滞作用更为明显。对SW480的增殖抑制效果明显提高(p<0.05)。结论:转染hTGF-β1基因可明显增强ALA-PDT对体外培养的结肠癌细胞SW480的增殖抑制作用。Objective：To study the effect of ALA PDT combined with human TGF- β1 gene transfection on proliferation of colon carcinoma SW480 cells. Methods：SW480 cells were infected with TGF - β1 - contained recombinant adenovirus, and treated with ALA - PDT. The expression of TGF - β1 was detected by immunohistechemical method. The proliferation of SW480 cells was detected by MTT assay. The C0 - G1 and S fraction were assayed by flow cytometry. Resuits： There was increased expression of TGE - β1 in SW480 cell line with transgenosis treatment at protein levels. When treated with ALA - PDT combined with TGF -β1 gene transfection, the G0 - G1 phase fraction increased remarkably, and the ability of cell proliferation decreased significantly in SW480 cell line, compared with that of PDT group（ p 〈 0. 05 ）. Conclusion ： The proliferation of the colon carcinoma SW480 cell line may inhibited significantly by ALA - PDT combined with transgenosis of hTGF - β1.

关 键 词：光动力疗法 转化生长因子Β1 结肠癌 增殖抑制 周期阻滞 

分 类 号：TN248.1[电子电信—物理电子学]