Optimization of Riboflavin Production from ccpA Mutant Bacillus subtilis 24A1/pMX45  

作　　者：应明 张帆 班睿 

机构地区：[1]School of Chemical Engineering and Technology, Tianjin University

出　　处：《Transactions of Tianjin University》2006年第6期415-419,共5页天津大学学报（英文版）

摘　　要：The nitrogen source requirements for riboflavin production by ccpA mutant Bacillus subtilis 24A1/pMX45 were optimized using linear regression. The optimal medium components considered included 8% glucose as carbon source, 2% yeast powder, 0.05% MgSO4 ·7H2O, and four types of nitrogen sources ： 0.1% yeast extract, 2% soybean powder, 1% corn plasm, and 0.2% （ NH4 ） 2 HPO4 in shake flask tests. Predictive ellipsoid was applied to determining the response values under the optimal levels for riboflavin production and glucose consumption. The optimal concentrations of the four types of nitrogen sources can remedy ammonium assimilative defection of ccpA mutant. Under the optimal conditions, the riboflavin yield increases to more than 5.0 g/L and 8%, glucose can be consumed completely after 60 h.The nitrogen source requirements for riboflavin production by ccpA mutant Bacillus subtilis 24A1/pMX45 were optimized using linear regression. The optimal medium components considered included 80/0 glucose as carbon source, 20/0 yeast powder, 0.050/0 MgSO4·7H2O, and four types of nitrogen sources: 0.10/0 yeast extract, 20/0 soybean powder, 10/0 corn plasm, and 0.20/0 （NH4）2HPO4 in shake flask tests. Predictive ellipsoid was applied to determining the response values under the optimal levels for riboflavin production and glucose consumption. The optimal concentrations of the four types of nitrogen sources can remedy ammonium assimilative defection of ccpA mutant. Under the optimal conditions, the riboflavin yield increases to more than 5.0 g/L and 80/0 glucose can be consumed completely after 60 h.

关 键 词：carbon catabolic repression （ CCR ） global regulator CcpA Bacillus subtilis orthogonal test linear regression 

分 类 号：TQ465[化学工程—制药化工]