高效液相色谱法测定小儿清肺止咳泡腾片中黄芩苷含量  

Determination of Baicalin in Xiaoer Qingfei Zhike Effervescent Tablet by HPLC

作　　者：常忆凌[1] 刘本[2] 

机构地区：[1]浙江医药高等专科学校,浙江宁波315100 [2]浙江大学宁波理工学院,浙江宁波315100

出　　处：《中国药业》2006年第20期21-22,共2页China Pharmaceuticals

基　　金：浙江省教育厅课题;课题编号:20020702

摘　　要：目的用高效液相色谱法(HPLC法)测定小儿清肺止咳泡腾片中黄芩苷含量。方法采用DiamonsilC18柱(250mm×4.6mm,5μm),流动相为甲醇-水-甲酸(50∶50∶0.2),检测波长为280nm,流速为1.2mL/min。结果黄芩苷进样量在0.344～3.44μg的范围内与峰面积线性关系良好。结论HPLC法快速、准确,专属性好,灵敏度高。Objective To set up a method of determination of balcalin in Xiaoer Qingfei Zhike effervescent tablet by HPLC. Methods Chromatographic coh,mn Diamonsil C18 （250 mm× 4.6 mm,5μm） was used, mobile phase was methanol -water -hrmic acid （50 ： 50：0. 2）, determination wavelength was 280 nm,and flow rate was 1.2 mL/min. Results The linear range of baicalin was 0. 344- 3.44μg. Conclusion This method is simple, accurate, highly sensitive and reproducible. It can be used for determination of Xiaoer Qingfei Zhike effervescent tablet.

关 键 词：高效液相色谱法 黄苓苷 小儿清肺止咳泡腾片 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]