蕨麻多糖对小鼠淋巴细胞花生四烯酸代谢的影响  

Effects of Potentilla anserine Polysaccharide on metabolic product of arachidonic acid from lymphocyte in mice

作　　者：程富胜[1] 胡庭俊[1] 张霞[2] 陈炅然[1] 孟聚诚[1] 高芳[1] 郑荣梁[3] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所新兽药工程重点实验室,甘肃兰州730050 [2]甘肃农业大学生命科学技术学院 [3]上海高校一氧化氮及炎症医学E-研究院

出　　处：《中兽医医药杂志》2006年第6期20-22,共3页Journal of Traditional Chinese Veterinary Medicine

基　　金：国家自然科学基金项目(30371056)

摘　　要：目的:探讨蕨麻多糖(PAP)对小鼠脾淋巴细胞内花生四烯酸(AA)代谢产物的影响。方法:用放射免疫方法检测了血栓素B2(TXB2)和6-酮-前列腺素F1α(6-K-PGF1α)在细胞内的含量。结果:不同蕨麻多糖浓度组,6-K-PGF1α含量达到高峰的时间段各不相同;与对照组相比较,100μg/mlPAP组TXB2的含量明显偏低,而其它各组在同一时间段TXB2含量与多糖浓度呈剂量依赖关系;并且PGF1α/TXB2比值也随PAP浓度递增而升高。结论:PAP能调节小鼠脾淋巴细胞AA代谢水平和机体PGF1α/TXB2平衡状态,影响机体免疫机能。Objective： To investigate the effects of potentilla anserine polysaccharide（PAP） on metabolic product of araehidonic acid （ AA ） in routine spienoeytes. Methods ： Thromboxane B2 （ TXB2 ） and 6 - Keto - prosta glandin F1α （ 6 - K - PGF1α ） from Cells were detected by radioimmunoassay （RIA）. Results： In the groups of different concentration of PAP, the content of 6 -K -PGF1α reached maximum at different time. Compared with the control, the content of TXB2 in the group of PAP by 100 μg/ml was decreased, but in the other groups, at the same period of time, the content showed dose - dependant manner, and the value of PGF1α/TXB2 was increased with the rising of concentration of PAP. Conclusion： The level of metabolic product of AA was changed by PAP, as well as PGF1α/TXB2 was adjusted, and immunological function was promoted.

关 键 词：蕨麻多糖 6—K—PGF1α TXB2 

分 类 号：S852.42[农业科学—基础兽医学]