HPLC法测定护肝丸中五味子醇甲的含量  

Content Determination of Schisandrin in Hugan Pill by HPLC

作　　者：章泽恒[1] 龙海艳 赵勇[1] 

机构地区：[1]湖南省药品检验所,湖南长沙410001

出　　处：《中国中医药信息杂志》2006年第12期56-57,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立高效液相色谱法测定五味子醇甲含量的方法。方法以反相高效液相色谱法测定五味子醇甲的含量。色谱柱:Hanbon Lichrospher C18(250mm×4.6mm,5μm);流动相:甲醇-水(63∶37);检测波长:251nm。结果五味子醇甲在0.1051～2.102μg浓度范围内线性关系良好(r=0.9999,n=7);平均加样回收率为97.03%,RSD为1.02%。结论该方法快速,简便,准确,重复性好。Objective To develop the method for determining the content of Schisandrin by HPLC. Method The Schisandrin in Hugan Pill was determinined by HPLC. The analytical column was Hanbon Lichrospher （250 mm×4.6 mm, 5 μm） and mobile phase was methanol-water （67 ： 33）, with wavelength was set at 251 nm. Result Schisandrin had good linear relationship with their peak area in the range of 0.105 1-2.102 μg （r=0.9999, n=7）. The average recovery rate was 97.03%, RSD was 1.02% （n=5）. Conclusion The method was rapid, simple and precise.

关 键 词：护肝丸 五味子醇甲 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]