肺型氧中毒小鼠肺组织巨噬细胞炎症蛋白-2 mRNA表达的改变  

Changes of macrophage inflammatory protein-2 mRNA expression in the lung tissue of pulmonary oxygen toxicity mice

作　　者：王世锋[1] 王文波[1] 吴苏舒[1] 

机构地区：[1]海军医学研究所,上海200433

出　　处：《中华航海医学与高气压医学杂志》2006年第4期239-242,共4页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的观察高压氧暴露后小鼠肺组织巨噬细胞炎症蛋白-2(macrophage inflammatory protein-2,MIP-2)mRNA表达的影响。方法40只健康C57BL／6雄性小鼠采用随机数字法分为5组: 650 UPTD组、850 UPTD组、1100 UPTD组、高压常氧组和对照组。小鼠在密闭氧舱中吸入高压纯氧建立肺型氧中毒模型。对照组吸入空气,高压常氧组吸入氮氧混合气,氧分压与空气中氧分压相同。动物出舱后解剖取肺,肺组织用于病理检测和RT-PCR方法检测MIP-2 mRNA的表达。结果(1)高压氧暴露组与对照组相比,MIP-2 mRNA表达明显增高(P<0．05);(2)高压常氧组与对照组MIP-2 mRNA表达没有明显变化(P>0．05)。结论随着高压氧暴露剂量的增加,MIP-2 mRNA的表达明显增多,并且炎症损伤也呈增加趋势。Objective To observe the effects of the expression of macrophage inflammatory protein-2 mRNA in the lung tissue of hyperbaric oxygen exposed mice. Methods The pulmonary oxygen toxicity model mice were duplicated by breathing hyperbaric oxygen, and the 40 mice were divided into 5 groups randomly：650 UPTD group, 850 UPTD group, 1 100 UPTD group, normal O2 group and control group. The control group mice breathed atmosphere, and the normal O2 group breathed N2-O2 mixed gas, in which O2 partial pressure was the same as that in atmosphere. The expression of MIP-2 mRNA in lung was measured by RT-PCR. Results The expressions of MIP-2 mRNA in HBO exposed groups were significantly higher than those in the control group （P 〈 0.05 ） , whereas the expression of MIP-2 mRNA of in normal O2 group did not show significant difference from that in the control group （ P 〉 0.05 ）. Conclusions Pulmonary oxygen toxicity may elevate the expression of MIP-2 mRNA in mice lung tissue, and the extent of inflammation may be relevant to the oxygen toxicity dose.

关 键 词：高压氧 肺型氧中毒 巨噬细胞炎症蛋白-2 

分 类 号：R595.1[医药卫生—内科学]